cells

Immature dendritic cells that take up antigen and later become antigen-presenting cells (APC) that activate T lymphocytes)

Cell Type:

Dendritic cell (DC)

Tissue Origin:

blood

Species:

mouse

Research Area:

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
P4	DK-100	5e4	29-39%	35-47%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
DC, mouse	Y-001	3e5	54% ±4	52% ±4	Plasmid (general)	2 µg	100 µl	I/II/2b
P4	96-DK-100	5e4	34% ±5	41% ±6	Plasmid (general)	0.4 µg	20 µl	96-well

Citations

Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9.

Categories:

Transfection, Cell Lines and Primary Cancer Cells

Authors:

Freund EC, Lock JY, Oh J, Maculins T, Delamarre L, Bohlen CJ, Haley B, Murthy A.

In:

J Exp Med (2020) 217(7): e20191692

Dendritic cell, mouse, immat. - C57BL/6

Transfection Information

Citations