Dendritic cell, mouse, immat. - C57BL/6

Immature dendritic cells that take up antigen and later become antigen-presenting cells (APC) that activate T lymphocytes)

Cell Type:
Dendritic cell (DC)
Tissue Origin:
blood
Species:
mouse
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P4

DK-100

5e4 29-39% 35-47% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
DC, mouse Y-001 3e5 54% ±4 52% ±4 Plasmid (general) 2 µg 100 µl I/II/2b
P4 96-DK-100 5e4 34% ±5 41% ±6 Plasmid (general) 0.4 µg 20 µl Shuttle