cells

leukemia cell (CML), EBV positive

Cell Type:

Leukocyte (unspec.)

Tissue Origin:

blood

Species:

human

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
R	A-023	5e6	8%	80%	Plasmid (general)	5 µg	100 µl	I/II/2b
R	G-016	5e6	13%	60%	Plasmid (general)	5 µg	100 µl	I/II/2b
R	T-016	5e6	36%	30%	Plasmid (general)	5 µg	100 µl	I/II/2b
R	T-016	5e6	19%	53%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

FoxO3a and BCR-ABL regulate cyclin D2 transcription through a STAT5/BCL6-dependent mechanism

Categories:

Transfection

Authors:

Fernandez de Mattos S, Essafi A, Soeiro I, Pietersen AM, Birkenkamp KU, Edwards CS, Martino A, Nelson BH, Francis JM, Jones MC, Brosens JJ, Coffer PJ and Lam EW

In:

Mol Cell Biol (2004) 24(22): 10058-10071

BV173

Transfection Information

Citations