BV173

leukemia cell (CML), EBV positive

Cell Type:
Leukocyte (unspec.)
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R T-016 5e6 19% 53% Plasmid (general) 2 µg 100 µl I/II/2b
R T-016 5e6 36% 30% Plasmid (general) 5 µg 100 µl I/II/2b
R G-016 5e6 13% 60% Plasmid (general) 5 µg 100 µl I/II/2b
R A-023 5e6 8% 80% Plasmid (general) 5 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Fernandez de Mattos S, Essafi A, Soeiro I, Pietersen AM, Birkenkamp KU, Edwards CS, Martino A, Nelson BH, Francis JM, Jones MC, Brosens JJ, Coffer PJ and Lam EW 
In:
Mol Cell Biol (2004) 24(22): 10058-10071