Hematopoietic stem cells, mouse
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hematopoetic stem cells, mouse, sca-1 positive; mouse CD34+
Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
bone marrow
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Stem Cells
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
P3
DK-100
2e5
75%
65%
Plasmid (general)
2 µg
20 µl
4D X-Unit
Citations
Generation of locus-specific degradable tag knock-ins in mouse and human cell lines
Categories:
Transfection
Authors:
Damhofer H, Radzisheuskaya A, Helin K.
In:
STAR Protoc (2021) 2(2): 100575
TALEN mediated gene editing in a mouse model of Fanconi anemia
Categories:
Classical Media and Reagents, Transfection
Authors:
Maria José Pino-Barrio , Yari Giménez, Mariela Villanueva , Marcus Hildenbeutel , Rebeca Sánchez-Dominguez , Sandra Rodríguez-Perales , Roser Pujol , Jordi Surrallés , Paula Río , Toni Cathomen , Claudio Mussolino , Juan Antonio Bueren , Susana Navarro
In:
Scientific Reports (2020) 10(1): 6997
Efficient CRISPR/Cas9-Mediated Gene Knockin in Mouse Hematopoietic Stem and Progenitor Cells
Categories:
Transfection
Authors:
Ngoc Tung Tran , Thomas Sommermann , Robin Graf , Janine Trombke , Jenniffer Pempe , Kerstin Petsch , Ralf Kühn , Klaus Rajewsky , Van Trung Chu
In:
Cell Rep (2019) 28(13): 3510-3522
Interferon-gamma impairs proliferation of hematopoietic stem cells in mice.
Categories:
Serum-free and Speciality Media
Authors:
de Bruin AM, Demirel Ö, Hooibrink B, Brandts CH, Nolte MA.
In:
Blood (2013) 121(18): 3578-85