Hematopoietic stem cells, mouse

hematopoetic stem cells, mouse, sca-1 positive; mouse CD34+

Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
bone marrow
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Stem Cells
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 DK-100 2e5 75% 65% Plasmid (general) 2 µg 20 µl 4D X-Unit

Citations

Categories:
Classical Media and Reagents, Transfection 
Authors:
Maria José Pino-Barrio , Yari Giménez, Mariela Villanueva , Marcus Hildenbeutel , Rebeca Sánchez-Dominguez , Sandra Rodríguez-Perales , Roser Pujol , Jordi Surrallés , Paula Río  , Toni Cathomen , Claudio Mussolino , Juan Antonio Bueren , Susana Navarro 
In:
Scientific Reports (2020) 10(1): 6997 
Categories:
Transfection 
Authors:
Ngoc Tung Tran , Thomas Sommermann , Robin Graf , Janine Trombke , Jenniffer Pempe , Kerstin Petsch , Ralf Kühn , Klaus Rajewsky , Van Trung Chu  
In:
Cell Rep (2019) 28(13): 3510-3522 
Categories:
Serum-free and Speciality Media 
Authors:
de Bruin AM, Demirel Ö, Hooibrink B, Brandts CH, Nolte MA. 
In:
Blood (2013) 121(18): 3578-85