Neuron, striatal, mouse

Neurons isolated from the striatum (subcortical part of the brain consisting of the caudate nucleus and the putamen. It is part of the basal ganglia and is best known for its role in the planning and modulation of movement pathways).

Cell Type:
Neurons (Brain)
Tissue Origin:
brain
Species:
mouse
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

Primary Neuron Growth Medium Kit (PNGM™) was specifically developed for culturing embryonic primary rat and mouse neurons. This serum-free medium allows for long-term maintenance and growth of neuronal cells, and maintains pure populations of neuronal cells without the need of an astrocyte feeder layer.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Neuron, mouse 4e6 75-85% Plasmid (general) µg 100 µl I/II/2b

Citations

Categories:
Primary Cells and Media 
Authors:
Garcia-Munoz M, Lopez-Huerta VG, Carrillo-Reid L, Arbuthnott GW 
In:
Other (2015) 89: 54-63 
Categories:
Primary Cells and Media 
Authors:
Nousiainen HO, Quintero IB, Myöhänen TT, Voikar V, Mijatovic J, Segerstråle M, Herrala AM, Kulesskaya N, Pulkka AE1, Kivinummi T, Abo-Ramadan U, Taira T, Piepponen TP, Rauvala H, Vihko P 
In:
PLoS ONE (2014) 9(5): ePub 
Categories:
Transfection 
Authors:
Jin K, LaFevre-Bernt M, Sun Y, Chen S, Gafni J, Crippen D, Logvinova A, Ross CA, Greenberg DA and Ellerby LM 
In:
Proc Natl Acad Sci USA (2005) 102(50): 18189-18194