CHO-S FreeStyle™
Export
sCHO clone adapted to serum-free suspension growth in FreeStyle™ CHO Expression Medium
Cell Type:
Ovary Epith.
Tissue Origin:
ovarian
Species:
hamster
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
DG-208
4e5
80-85%
50%
Plasmid (general)
0.8 µg
20 µl
4D X-Unit
SF
DG-208
2e6
81-83%
59-61%
Plasmid (general)
2 µg
100 µl
4D X-Unit
Citations
Recombinant Ig-Like Transcript 3-Fc Modulates T Cell Responses via Induction of Th Anergy and Differentiation of CD8+ T Suppressor Cells
Categories:
Transfection
Authors:
Kim-Schulze S, Scotto L, Vlad G, Piazza F, Lin H, Liu Z, Cortesini R, Suciu-Foca N
In:
J Immunol (2006) 176(5): 2790-8