BLCL
Export
EBV-transformed B cell line, B-Lymphoblastoid Cell Line
Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
C
Z-001
2e6
80%
80%
Plasmid (general)
2 µg
100 µl
I/II/2b
C
X-005
2e6
77%
70%
Plasmid (general)
2 µg
100 µl
I/II/2b
C
Y-001
2e6
67%
80%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Highly efficient induced pluripotent stem cell reprogramming of cryopreserved lymphoblastoid cell lines
Categories:
Transfection
Authors:
Satish Kumar , Joanne E Curran , Erika C Espinosa , David C Glahn , John Blangero
In:
J Bio Med Opt (2020) 7(1): 124
Cross-recognition of avian H5N1 influenza virus by human cytotoxic T lymphocyte populations directed to human influenza A virus
Categories:
Transfection
Authors:
Kreijtz J, de Mutsert G, van Baalen C, Fouchier R, Osterhaus A, Rimmelzwaan G
In:
J Virol (2008) 82(11): 5161-6
The loss of immunodominant epitopes affects interferon-gamma production and lytic activity of the human influenza virus-specific cytotoxic T lymphocyte response in vitro
Categories:
Transfection
Authors:
Berkhoff EG, Geelhoed-Mieras MM, Verschuren EJ, van Baalen CA, Gruters RA, Fouchier RA, Osterhaus AD, Rimmelzwaan GF
In:
Clin Exp Immunol (2007) 148(2): 296-306
Recognition of Breast Cancer Cells by CD8+ Cytotoxic T-Cell Clones Specific for NY-BR-1
Categories:
Transfection
Authors:
Wang W, Epler J, Salazar LG, Riddell SR
In:
Cancer Res (2006) 66(13): 6826-33
Fluorescent antigen-transfected target cell cytotoxic T lymphocyte assay for ex vivo detection of antigen-specific cell-mediated cytotoxicity
Categories:
Transfection
Authors:
van Baalen CA, Kwa D, Verschuren EJ, Reedijk ML, Boon AC, de Mutsert G, Rimmelzwaan GF, Osterhaus AD and Gruters RA
In:
J Infect Dis (2005) 192(7): 1183-1190