Fibroblast, cardiac, rat

Fibroblasts isolated from the heart

Cell Type:
Fibroblast
Tissue Origin:
heart
Species:
rat
Research Area:
Cardiovascular
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Fibroblast, basic U-023 85% moderate Plasmid (general) µg 100 µl I/II/2b
Fibroblast, basic T-016 30% low Plasmid (general) µg 100 µl I/II/2b
AD2 EH-158 3e5 20-60%
Plasmid (general) 16 µg 350 µl 4D Y-Unit