Mesench. stem (MSC), pig

bone marrow derived stem cells with the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. Generally considered a subpopulation of bone marrow stromal cells, although often the terms are used interchangeably.

Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
Species:
porcine
Research Area:
Cancer Research/Cell Biology
Stem Cells
Cell Characteristics:
Adherent

Recommended Media

TheraPEAK™ MSCGM™ Mesenchymal Stem Cell Growth Medium, Serum-free (with L-glutamine, without phenol red and antibiotics).

TheraPEAK™MSCGM™ is a serum free medium for the growth of human Mesenchymal Stem Cells. TheraPEAK™ MSCGM™ is optimized for the multiple passage expansion of all types of hMSCs while still allowing for differentiation into the several desired lineages. Cells can be directly transitioned from serum-containing medium to TheraPEAK™ MSCGM™ with little to no adaptation time. For peak performance, it is recommended to use TheraPEAK™ MSCGM™ with Fibronectin coated plates

UltraCULTURE™ Medium is a complete all purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines.
The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution (Cat. No. 17-605) per 500 ml.

The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.

UltraCULTURE™ Medium may be supplemented with Cryoprotective Medium (Cat. No. 12-132) to cryopreserve cells in a serum-free environment.

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
MSC, human G-022 2e6 60% 50% Plasmid (general) 10 µg 100 µl I/II/2b
P1 EH-100 4e5 83% 82% Plasmid (general) 1 µg 4D X-Unit
P1 FF-113 4e5 83% 68% Plasmid (general) 1 µg 4D X-Unit

Citations

Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Izuagie Attairu Ikhapoh, Christopher J Pelham and Devendra K Agrawal 
In:
Stem Cell Res Ther (2015) 6(4): 1186 
Categories:
Transfection 
Authors:
Sheyn D, Pelled G, Zilberman Y, Talasazan F, Frank JM, Gazit D, Gazit Z 
In:
Stem Cells (2008) 26(4): 1056-64