Mesench. stem (MSC), pig

bone marrow derived stem cells with the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. Generally considered a subpopulation of bone marrow stromal cells, although often the terms are used interchangeably.

Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
Species:
porcine
Research Area:
Cancer Research/Cell Biology
Stem Cells
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
MSC, human G-022 2e6 60% 50% Plasmid (general) 10 µg 100 µl I/II/2b
P1 EH-100 4e5 83% 82% Plasmid (general) 1 µg 4D X-Unit
P1 FF-113 4e5 83% 68% Plasmid (general) 1 µg 4D X-Unit

Citations

Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Izuagie Attairu Ikhapoh, Christopher J Pelham and Devendra K Agrawal 
In:
Stem Cell Res Ther (2015) 6(4): 1186 
Categories:
Transfection 
Authors:
Sheyn D, Pelled G, Zilberman Y, Talasazan F, Frank JM, Gazit D, Gazit Z 
In:
Stem Cells (2008) 26(4): 1056-64