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Thyroid medullary carcinoma cells

Cell Type:
Thyroid Epith.
Tissue Origin:
thyroid
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

Ham's F12 Medium is a nutrient mixture designed to cultivate a wide variety of mammalian and hybridoma cells (including low density (clonal) growth of CHO cells) when used with serum in combination with hormones and transferrin.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
L M-029 2e6 60% 39.5% Plasmid (general) 2 µg 100 µl I/II/2b
L L-029 2e6 47.5% 60% Plasmid (general) 2 µg 100 µl I/II/2b
L A-030 2e6 45.2% 60% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Gutierrez JA, Solenberg PJ, Perkins DR, Willency JA, Knierman MD, Jin Z, Witcher DR, Luo S, Onyia JE, Hale JE 
In:
Proc Natl Acad Sci USA (2008) 105(17): 6320-5