NTERA-2 cl.D1

A pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line. The parental NTERA-2 lines was established in 1980 from a nude mouse xenograft of the Tera-2 cell line.
Cell Type:
Testes Epith.
Tissue Origin:
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
L X-001 1e6 90% ±2 94% ±1 Plasmid (general) 2 µg 100 µl I/II/2b
SF CA-137 2e5 82% ±1 72% ±2 Plasmid (general) 0.6 µg 20 µl 4D X-Unit
SF CA-137 1e6 84% 70% Plasmid (general) 3 µg 100 µl 4D X-Unit