cells

An interleukin-2 (IL-2) dependent Natural Killer Cell line derived from peripheral blood mononuclear cells from a 50 year old Caucasian male with rapidly progressive non-Hodgkin's lymphoma.

Cell Type:

Natural Killer (NK)

Tissue Origin:

blood

Species:

human

Research Area:

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Recommended Media

MEM Alpha Eagle

MEM Alpha was developed in 1971 and differs from standard MEM by containing the following unique components: vitamin B12, ascorbic acid, non-essential amino acids, sodium pyruvate, lipoic acid, and D-biotin.
This media has been used to culture bone marrow cells and amniotic cells for chromosome analysis.

MEM Alpha is available as:
12-169 - without L-glutamine, deoxyribonucleosides or ribonucleosides
BE02-002 - with Ultraglutamine, deoxyribonucleosides and ribonucleosides

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SE	CA-137	1e6	39.9%	66.9%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SE	CA-137	5e6	45.2%	71.9%	Plasmid (general)	2 µg	100 µl	4D X-Unit
R	A-024	5e6	16-34%	30-50%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations (9)

Virus-free CRISPR knockin of a chimeric antigen receptor into KLRC1 generates potent GD2-specific natural killer cells

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection

Authors:

Shankar K, Zingler-Hoslet I, Tabima DM, Zima S, Shi L, Gimse K, Forsberg MH, Katta V, Davis SZ, Maldonado D, Russell BE, Murtaza M, Tsai SQ, Ayuso JM, Capitini CM, Saha K

In:

Mol Ther (2025) 33(3): 1014-1030

Integration of ?-deficient CARs into the CD3-zeta gene conveys potent cytotoxicity in T and NK cells

Categories:

Transfection, Cell Lines and Primary Cancer Cells

Authors:

Kath J, Franke C, Drosdek V, Du W, Glaser V, Fuster-Garcia C, Stein M, Zittel T, Schulenberg S, Porter CE, Andersch L, Künkele A, Alcaniz J, Hoffmann J, Abken H, Abou-El-Enein M, Pruß A, Suzuki M, Cathomen T, Stripecke R, Volk HD, Reinke P, Schmueck-Henneresse M, Wagner DL

In:

Blood (2024) 143(25): :2599-2611

Protocol for genomic editing in human resting primary NK cells and NK-92 cells via CRISPR-Cas9 ribonucleoproteins

Categories:

Transfection

Authors:

Verhezen T, Lau HW, Van Audenaerde J, Wouters A, Smits E, De Waele J

In:

STAR Protoc (2024) 5(3): 103123

Dose intensification of TRAIL-inducing ONC201 inhibits metastasis and promotes intratumoral NK cell recruitment.

Categories:

Live Cell Imaging

Authors:

Wagner J1, Kline CL1, Zhou L1, Campbell KS2, MacFarlane AW2, Olszanski AJ3, Cai KQ4, Hensley HH5, Ross EA6, Ralff MD1, Zloza A7, Chesson CB7, Newman JH7, Kaufman H7, Bertino J7, Stein M7, El-Deiry WS1

In:

J Clin Invest (2018) 6: 2325-2338

Clinical grade manufacturing of genetically modified, CAR-expressing NK-92 cells for the treatment of ErbB2-positive malignancies.

Categories:

Serum-free and Speciality Media

Authors:

Nowakowska P, Romanski A, Miller N, Odendahl M, Bonig H, Zhang C, Seifried E, Wels WS, Tonn T

In:

Cancer Immunol Immunother (2018) 67 (1): 25-38

Selective inhibition of tumor growth by clonal NK cells expressing an ErbB2/HER2-specific chimeric antigen receptor.

Categories:

Serum-free and Speciality Media

Authors:

Schönfeld K, Sahm C, Zhang C, Naundorf S, Brendel C, Odendahl M, Nowakowska P, Bönig H, Köhl U, Kloess S, Köhler S, Holtgreve-Grez H, Jauch A, Schmidt M, Schubert R, Kühlcke K, Seifried E, Klingemann HG, Rieger MA, Tonn T, Grez M, Wels WS.

In:

Mol Ther (2015) 23 (2): 330-8

Comprehensive epigenetic profiling identifies multiple distal regulatory elements directing transcription of the gene encoding interferon-gamma

Categories:

Transfection

Authors:

Schoenborn JR, Dorschner MO, Sekimata M, Santer DM, Shnyreva M, Fitzpatrick DR, Stamatoyonnapoulos JA, Wilson CB

In:

Nat Immunol (2007) 8(7): 732-42

Many NK cell receptors activate ERK2 and JNK1 to trigger microtubule organizing center and granule polarization and cytotoxicity

Categories:

Transfection

Authors:

Chen X, Trivedi PP, Ge B, Krzewski K, Strominger JL

In:

Proc Natl Acad Sci USA (2007) 104(15): 6329-34

A Novel NF-B Binding Site Controls Human Granzyme B Gene Transcription

Categories:

Transfection

Authors:

Huang C, Bi E, Hu Y, Deng W, Tian Z, Dong C, Hu Y, Sun B

In:

J Immunol (2006) 176(7): 4173-81

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