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Mus Musculus embryonal fibroblast, fibroblast-like before differentiation, adipocyte-like after differentiation.

Cell Type:
Fibroblast
Tissue Origin:
embryo
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE CA-133 8e5 26% 50% Plasmid (general) 0.8 µg 20 µl 4D X-Unit
SE CA-133 4e6 30% 29-43% Plasmid (general) 4 µg 100 µl 4D X-Unit
L

A-033

 2e6 17-33% 67-100% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (15)

A biomimetic hydrogel functionalized with adipose ECM components as a microenvironment for the 3D culture of human and murine adipocytes.
Categories:
3D Cell Culture  
Authors:
Louis F, Pannetier P, Souguir Z, Le Cerf D, Valet P, Vannier JP, Vidal G, Demange E 
In:
Biotechnol Bioeng (2017) 114(8): 1813-1824 
Thyroid hormone receptor sumoylation is required for preadipocyte differentiation and proliferation.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Liu YY, Ayers S, Milanesi A, Teng X, Rabi S, Akiba Y, Brent GA 
In:
J Biol Chem (2015) 290(12): 7402-15 
Anti-Adipogenic Polyphenols of Water Shield Suppress TNF-a-Induced Cell Damage and Enhance Expression of HAS2 and HAPB2 in Adiponectin- Treated Dermal Fibroblasts
Categories:
Primary Cells and Media 
Authors:
Shimoda1 H, Nakamura S, Hitoe S, Terazawa S, Tanaka J, Matsumoto T, Matsuda H 
In:
Other (2014) 2(5): 1-7 
Antiadipogenic effects of the mineralocorticoid receptor antagonist drospirenone: potential implications for the treatment of metabolic syndrome.
Categories:
Primary Cells and Media 
Authors:
Caprio M, Antelmi A, Chetrite G, Muscat A, Mammi C, Marzolla V, Fabbri A, Zennaro MC, Fève B 
In:
Endocrinology (2011) 152(1): 113-125 
Down-regulation of tissue inhibitor of metalloproteinase-3 (TIMP-3) expression is necessary for adipocyte differentiation.
Categories:
Primary Cells and Media, Transfection 
Authors:
Bernot D, Barruet E, Poggi M, Bonardo B, Alessi MC, Peiretti F. 
In:
J Biol Chem (2010) 285(9): 6508-14 
Angiotensin-converting enzyme (ACE) inhibitors modulate cellular retinol-binding protein 1 and adiponectin expression in adipocytes via the ACE-dependent signaling cascade.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kohlstedt K, Gershome C, Trouvain C, Hofmann WK, Fichtlscherer S, Fleming I. 
In:
Mol Pharmacol (2009) 75(3): 685-92 
Hypoxia is a potential risk factor for chronic inflammation and adiponectin reduction in adipose tissue of ob/ob and dietary obese mice
Categories:
Transfection 
Authors:
Ye J, Gao Z, Yin J, He Q 
In:
Am J Physiol Endocrinol Metab (2007) 293(4): E1118-28 
Genetic and pharmacological inhibition of Rho-associated kinase II enhances adipogenesis
Categories:
Transfection 
Authors:
Noguchi M, Hosoda K, Fujikura J, Fujimoto M, Iwakura H, Tomita T, Ishii T, Arai N, Hirata M, Ebihara K, Masuzaki H, Itoh H, Narumiya S, Nakao K 
In:
J Biol Chem (2007) 282(40): 29574-83 
A Widely Used Retinoic Acid Receptor Antagonist Induces PPAR Activity
Categories:
Transfection 
Authors:
Schupp M, Curtin JC, Kim RJ, Billin AN, Lazar MA 
In:
Mol Pharmacol (2007) 71(5): 1251-7 
Insulin stimulates membrane fusion and GLUT4 accumulation in clathrin coats on adipocyte plasma membranes
Categories:
Transfection 
Authors:
Huang S, Lifshitz LM, Jones C, Bellve KD, Standley C, Fonseca S, Corvera S, Fogarty KE, Czech MP 
In:
Mol Cell Biol (2007) 27(9): 3456-3469 
Impaired adipogenesis caused by a mutated thyroid hormone alpha1 receptor
Categories:
Transfection 
Authors:
Ying H, Araki O, Furuya F, Kato Y, Cheng SY 
In:
Mol Cell Biol (2007) 27(6): 2359-71 
A critical role for Ebf1 and Ebf2 in the adipogenic transcriptional cascade
Categories:
Transfection 
Authors:
Jimenez MA, Akerblad P, Sigvardsson M, Rosen ED 
In:
Mol Cell Biol (2007) 27(2): 743-757 
Role of the Toll-like Receptor 4/NF-B Pathway in Saturated Fatty Acid-induced Inflammatory Changes in the Interaction Between Adipocytes and Macrophages
Categories:
Transfection 
Authors:
Suganami T, Tanimoto-Koyama K, Nishida J, Itoh M, Yuan X, Mizuarai S, Kotani H, Yamaoka S, Miyake K, Aoe S, Kamei Y, Ogawa Y 
In:
Arterioscler Thromb Vasc Biol (2007) 27(1): 84-91 
Transcription Factor Activating Enhancer-binding Protein-2beta: A NEGATIVE REGULATOR OF ADIPONECTIN GENE EXPRESSION
Categories:
Transfection 
Authors:
Ikeda K, Maegawa H, Ugi S, Tao Y, Nishio Y, Tsukada S, Maeda S, Kashiwagi A 
In:
J Biol Chem (2006) 281(42): 31245-31253 
Impact of SRC homology 2-containing inositol 5'-phosphatase 2 gene polymorphisms detected in a Japanese population on insulin signaling
Categories:
Transfection 
Authors:
Kagawa S, Sasaoka T, Yaguchi S, Ishihara H, Tsuneki H, Murakami S, Fukui K, Wada T, Kobayashi S, Kimura I and Kobayashi M 
In:
J Clin Endocrinol Metab (2005) 90(5): 2911-2919 
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