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Mus Musculus embryonal fibroblast, fibroblast-like before differentiation, adipocyte-like after differentiation.

Cell Type:
Fibroblast
Tissue Origin:
embryo
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

Dulbecco's Modified Eagle Medium (DMEM) was developed in 1969 and is a modification of Basal Medium Eagle (BME) that differs from BME and MEM by the following characteristics:

  • Vitamins 4X greater than MEM. Vitamins and amino acids greater than BME
  • Types and quantities of amino acids greater than MEM and BME
  • Iron (ferric nitrate)

It is used in a wide range of mammalian cell culture applications.  The high glucose version is well suited to high density suspension culture. The low glucose formula is used for adherent dependent cells.

Storage = 2ºC to 8ºC

Liquid:
12-614 = 4.5 g/L glucose, without L-glutamine
12-604 = 4.5 g/L glucose, with L-glutamine
BE12-604/U1 = 4.5 g/L glucose, with Ultraglutamine I
12-914 = 4.5 g/L glucose, without L-glutamine (hybridoma screened)
12-917 = 4.5 g/L glucose, without L-glutamine or phenol red
12-709 = 4.5 g/L glucose and 25 mM HEPES, without L-glutamine
12-733= 4.5 g/L glucose, without L-glutamine or sodium pyruvate
12-741 = 4.5 g/L glucose, with L-glutamine, without sodium pyruvate

12-707 = 1.0 g/L glucose, without L-glutamine
12-708 = 1.0 g/L glucose and 25 mM HEPES, without L-glutamine

Powder:
15-614 = 4.5 g/L glucose, without L-glutamine or sodium pyruvate
15-604 = 4.5 g/L glucose, with L-glutamine or sodium pyruvate

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE CA-133 8e5 26% 50% Plasmid (general) 0.8 µg 20 µl 4D X-Unit
SE CA-133 4e6 30% 29-43% Plasmid (general) 4 µg 100 µl 4D X-Unit
L

A-033

2e6 17-33% 67-100% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (15)

Categories:
3D Cell Culture  
Authors:
Louis F, Pannetier P, Souguir Z, Le Cerf D, Valet P, Vannier JP, Vidal G, Demange E 
In:
Biotechnol Bioeng (2017) 114(8): 1813-1824 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Liu YY, Ayers S, Milanesi A, Teng X, Rabi S, Akiba Y, Brent GA 
In:
J Biol Chem (2015) 290(12): 7402-15 
Categories:
Primary Cells and Media 
Authors:
Shimoda1 H, Nakamura S, Hitoe S, Terazawa S, Tanaka J, Matsumoto T, Matsuda H 
In:
Other (2014) 2(5): 1-7 
Categories:
Primary Cells and Media 
Authors:
Caprio M, Antelmi A, Chetrite G, Muscat A, Mammi C, Marzolla V, Fabbri A, Zennaro MC, Fève B 
In:
Endocrinology (2011) 152(1): 113-125 
Categories:
Primary Cells and Media, Transfection 
Authors:
Bernot D, Barruet E, Poggi M, Bonardo B, Alessi MC, Peiretti F. 
In:
J Biol Chem (2010) 285(9): 6508-14 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kohlstedt K, Gershome C, Trouvain C, Hofmann WK, Fichtlscherer S, Fleming I. 
In:
Mol Pharmacol (2009) 75(3): 685-92 
Categories:
Transfection 
Authors:
Ye J, Gao Z, Yin J, He Q 
In:
Am J Physiol Endocrinol Metab (2007) 293(4): E1118-28 
Categories:
Transfection 
Authors:
Noguchi M, Hosoda K, Fujikura J, Fujimoto M, Iwakura H, Tomita T, Ishii T, Arai N, Hirata M, Ebihara K, Masuzaki H, Itoh H, Narumiya S, Nakao K 
In:
J Biol Chem (2007) 282(40): 29574-83 
Categories:
Transfection 
Authors:
Schupp M, Curtin JC, Kim RJ, Billin AN, Lazar MA 
In:
Mol Pharmacol (2007) 71(5): 1251-7 
Categories:
Transfection 
Authors:
Huang S, Lifshitz LM, Jones C, Bellve KD, Standley C, Fonseca S, Corvera S, Fogarty KE, Czech MP 
In:
Mol Cell Biol (2007) 27(9): 3456-3469 
Categories:
Transfection 
Authors:
Ying H, Araki O, Furuya F, Kato Y, Cheng SY 
In:
Mol Cell Biol (2007) 27(6): 2359-71 
Categories:
Transfection 
Authors:
Jimenez MA, Akerblad P, Sigvardsson M, Rosen ED 
In:
Mol Cell Biol (2007) 27(2): 743-757 
Categories:
Transfection 
Authors:
Suganami T, Tanimoto-Koyama K, Nishida J, Itoh M, Yuan X, Mizuarai S, Kotani H, Yamaoka S, Miyake K, Aoe S, Kamei Y, Ogawa Y 
In:
Arterioscler Thromb Vasc Biol (2007) 27(1): 84-91 
Categories:
Transfection 
Authors:
Ikeda K, Maegawa H, Ugi S, Tao Y, Nishio Y, Tsukada S, Maeda S, Kashiwagi A 
In:
J Biol Chem (2006) 281(42): 31245-31253 
Categories:
Transfection 
Authors:
Kagawa S, Sasaoka T, Yaguchi S, Ishihara H, Tsuneki H, Murakami S, Fukui K, Wada T, Kobayashi S, Kimura I and Kobayashi M 
In:
J Clin Endocrinol Metab (2005) 90(5): 2911-2919 
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