U266

Export

multiple myeloma

Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

Iscove's Modified Dulbecco's Medium

Iscove's Modified Dulbecco's Medium (IMDM) is a modified DME containing high glucose (4,500 mg/L), sodium pyruvate, additional amino acids, HEPES buffer (in liquid form), selenium and other components. IMDM is suitable for fast growing cells and can support the growth of a wide variety of mammalian cells.

Storage = 2ºC to 8ºC

12-722 with L-glutamine
12-726 without L-glutamine
12-915 with L-glutamine and screened to support hybridoma growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R T-001 2e6 30% 85% Plasmid (general) 5 µg 100 µl I/II/2b
R U-001 2e6 79% 74% Plasmid (general) 4 µg 100 µl I/II/2b
R T-001 2e6 72% 74% Plasmid (general) 4 µg 100 µl I/II/2b
R U-005 2e6 91% 60% Plasmid (general) 4 µg 100 µl I/II/2b
R U-023 2e6 89% 48% Plasmid (general) 4 µg 100 µl I/II/2b
R U-009 2e6 90% 51% Plasmid (general) 4 µg 100 µl I/II/2b

Citations (13)

CD56 expression in human myeloma cells derived from the neurogenic gene expression: possible role of the SRY-HMG box gene, SOX4.
Categories:
Transfection 
Authors:
Iqbal MS, Otsuyama K, Shamsasenjan K, Asaoku H, Kawano MM. 
In:
Int Immunol (2010) 91: 267-75 
The novel polyamine analogue CGC-11093 enhances the antimyeloma activity of bortezomib
Categories:
Transfection 
Authors:
Carew JS, Nawrocki ST, Reddy VK, Bush D, Rehg JE, Goodwin A, Houghton JA, Casero RA Jr, Marton LJ, Cleveland JL 
In:
Cancer Res (2008) 68(12): 4783-90 
Antimyeloma effects of a sesquiterpene lactone parthenolide
Categories:
Transfection 
Authors:
Suvannasankha A, Crean CD, Shanmugam R, Farag SS, Abonour R, Boswell HS, Nakshatri H 
In:
Clin Cancer Res (2008) 14(6): 1814-22 
G1P3, an IFN-induced survival factor, antagonizes TRAIL-induced apoptosis in human myeloma cells
Categories:
Transfection 
Authors:
Cheriyath V, Glaser KB, Waring JF, Baz R, Hussein MA, Borden EC 
In:
J Clin Invest (2007) 117(10): 3107-17 
MEK1/2 inhibitors potentiate UCN-01 lethality in human multiple myeloma cells through a Bim-dependent mechanism
Categories:
Transfection 
Authors:
Pei XY, Dai Y, Tenorio S, Lu J, Harada H, Dent P, Grant S 
In:
Blood (2007) 110(6): 2092-101 
Ursolic Acid Inhibits STAT3 Activation Pathway Leading to Suppression of Proliferation and Chemosensitization of Human Multiple Myeloma Cells
Categories:
Transfection 
Authors:
Pathak AK, Bhutani M, Nair AS, Ahn KS, Chakraborty A, Kadara H, Guha S, Sethi G, Aggarwal BB 
In:
Mol Cancer Res (2007) 5(9): 943-55 
Noxa Up-regulation and Mcl-1 Cleavage Are Associated to Apoptosis Induction by Bortezomib in Multiple Myeloma
Categories:
Transfection 
Authors:
Gomez-Bougie P, Wuillème-Toumi S, Ménoret E, Trichet V, Robillard N, Philippe M, Bataille R, Amiot M 
In:
Cancer Res (2007) 67(11): 5418-24 
Targeting aurora kinases as therapy in multiple myeloma
Categories:
Transfection 
Authors:
Shi Y, Reiman T, Li W, Maxwell CA, Sen S, Pilarski L, Daniels TR, Penichet ML, Feldman R, Lichtenstein A 
In:
Blood (2007) 109(9): 3915-21 
Dissecting the Roles of Checkpoint Kinase 1/CDC2 and Mitogen-Activated Protein Kinase Kinase 1/2/Extracellular Signal-Regulated Kinase 1/2 in Relation to 7-Hydroxystaurosporine-Induced Apoptosis in Human Multiple Myeloma Cells
Categories:
Transfection 
Authors:
Pei XY, Li W, Dai Y, Dent P, Grant S 
In:
Mol Pharmacol (2006) 70(6): 1965-1973 
Caspase-12 and caspase-4 are not required for caspase-dependent endoplasmic reticulum stress-induced apoptosis
Categories:
Transfection 
Authors:
Obeng EA and Boise LH 
In:
J Biol Chem (2005) 280(33): 29578-29587 
The FA/BRCA pathway is involved in melphalan-induced DNA interstrand cross-link repair and accounts for melphalan resistance in multiple myeloma cells
Categories:
Transfection 
Authors:
Chen Q, Van der Sluis PC, Boulware D, Hazlehurst LA and Dalton WS 
In:
Blood (2005) 106(2): 698-705 
The farnesyltransferase inhibitor L744832 potentiates UCN-01-induced apoptosis in human multiple myeloma cells
Categories:
Transfection 
Authors:
Pei XY, Dai Y, Rahmani M, Li W, Dent P and Grant S 
In:
Clin Cancer Res (2005) 11(12): 4589-4600 
HSP27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance
Categories:
Transfection 
Authors:
Chauhan D, Li G, Hideshima T, Podar K, Mitsiades C, Midsiades N, Catley L, Tai YT, Hayashi T, Shringharpure R, Burger R, Munshi N, Ohtake Y, Saxena S and Anderson KC 
In:
Blood (2003) 102(9): 3379-3386 
+ Show All

Privacy | Legal | About Lonza

©2023 Lonza. All rights reserved.