RIN m5f
Export
islet of Langerhans cells
Cell Type:
Pancreas
Tissue Origin:
pancreas
Species:
rat
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
DE-130
1e6
68% ±1
72% ±5
Plasmid (general)
1.2 µg
100 µl
4D X-Unit
SF
DE-130
2e5
90%
85% ±3
Plasmid (general)
600 ng
20 µl
4D X-Unit
SF
DE-130
2e5
90%
85% ±3
Plasmid (general)
600 ng
20 µl
96-well
Citations
Endogenous and synthetic agonists of GPR119 differ in signalling pathways and their effects on insulin secretion in MIN6c4 insulinoma cells
Categories:
Transfection
Authors:
Ning Y, O'Neill K, Lan H, Pang L, Shan LX, Hawes BE, Hedrick JA
In:
Br J Pharmacol (2008) epub: online
PGJ2 stimulated beta-cell apoptosis is associated with prolonged UPR activation
Categories:
Transfection
Authors:
Chambers KT, Weber SM, Corbett JA
In:
Am J Physiol Endocrinol Metab (2007) 292(4): E1052-61