RBL-2H3

Leukemia, Basophil

Cell Type:
Granulocyte
Tissue Origin:
blood
Species:
rat
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF DS-150 2e5 78% ±11 76% ±28 Plasmid (general) 1 µg 20 µl 4D X-Unit
SF DS-150 1e6 73% ±14 73% ±10 Plasmid (general) 2 µg 4D X-Unit
T X-001 1e6 42% ±2 93% ±2 Plasmid (general) 5 µg 100 µl I/II/2b

Citations (8)

Categories:
Transfection 
Authors:
Higashio H, Nishimura N, Ishizaki H, Miyoshi J, Orita S, Sakane A, Sasaki T 
In:
J Immunol (2008) 180(7): 4774-84 
Categories:
Transfection 
Authors:
Zhang J, Suzuki K, Hitomi T, Siraganian RP 
In:
J Biol Chem (2007) 282(52): 37669-77 
Categories:
Transfection 
Authors:
Tshori S, Sonnenblick A, Yannay-Cohen N, Kay G, Nechushtan H, Razin E 
In:
Mol Cell Biol (2007) 27(11): 3911-9 
Categories:
Transfection 
Authors:
Csutora P, Zarayskiy V, Peter K, Monje F, Smani T, Zakharov SI, Litvinov D, Bolotina VM 
In:
J Biol Chem (2006) 281(46): 34926-35 
Categories:
Transfection 
Authors:
Fattakhova G, Masilamani M, Borrego F, Gilfillan AM, Metcalfe DD, Coligan JE 
In:
Traffic (2006) 7(6): 673-85 
Categories:
Transfection 
Authors:
Levy C, Lee YN, Nechushtan H, Schueler-Furman O, Sonnenblick A, Hacohen S and Razin E 
In:
Blood (2006) 107(7): 2839-45 
Categories:
Transfection 
Authors:
Gaudreault E, Thompson C, Stankova J and Rola-Pleszczynski M 
In:
J Immunol (2005) 174(6): 3617-3625 
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