cells

Murine principal kidney cortical collecting duct cell line isolated from 1-mo-old SV-PK/Tag male mouse [Bens et al. (1999) J Am Soc Nephrol 10:923-934]. Express typical electrophysiologic features of tight epithelium.

Cell Type:

Kidney Epith.

Tissue Origin:

kidney

Species:

mouse

Research Area:

Gastroenterology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
L	T-020	2e6	60-70%	very good	Plasmid (general)	2 µg	100 µl	I/II/2b
L	T-030	2e6	70-80%	good	Plasmid (general)	2 µg	100 µl	I/II/2b
L	X-005	2e6	90-95%	good	Plasmid (general)	2 µg	100 µl	I/II/2b
SF	EN-138		70-80%	good	Plasmid (general)	0.4 µg		4D X-Unit
SF	EN-158		98%	high	Plasmid (general)	1 µg		4D X-Unit

Citations

Phosphatidylinositol 4-phosphate 5-kinase reduces cell surface expression of the epithelial sodium channel (ENaC) in cultured collecting duct cells

Categories:

Transfection

Authors:

Weixel KM, Edinger RS, Kester L, Guerriero CJ, Wang H, Fang L, Kleyman TR, Welling PA, Weisz OA, Johnson JP

In:

J Biol Chem (2007) 282(50): 36534-42

Differential Activities of Glucocorticoid-induced Leucine Zipper Protein Isoforms

Categories:

Transfection

Authors:

Soundararajan R, Wang J, Melters DP, Pearce D

In:

J Biol Chem (2007) 282(50): 36303-13

A Novel Role for Glucocorticoid-induced Leucine Zipper Protein in Epithelial Sodium Channel-mediated Sodium Transport

Categories:

Transfection

Authors:

Soundararajan R, Zhang TT, Wang J, Vandewalle A and Pearce D

In:

J Biol Chem (2005) 280(48): 39970-39981

mpkCCD(c14)

Transfection Information

Citations