Cell line derived from IOSE-80 (human ovarian surface epithelial cell immortalized by SV40 large T and small t antigens) by transformation with hTERT. Liu et al. (2004) Cancer Res. 64: 1655-1663.
Medium 199 was developed in 1950 and was formulated for nutritional studies on chick embryo muscle fibroblasts. Its unique components include purines (adenine, guanine, xanthine, hypoxanthine), pyrimdines (thymine, uracil) and fat soluble compounds. Its current uses include virology and vaccine production.These formulations require a CO2 atmosphere.Storage = 2ºC to 8ºC12-109 - with Hank's BSS, L-glutamine, and 1.4 g/L sodium bicarbonate12-118 - with Hank's BSS, L-glutamine, HEPES, and 1.4 g/L sodium bicarbonate12-117 - with Earle's BSS, L-glutamine, HEPES, and 2.2 g/L sodium bicarbonate12-119 - with Earle's BSS, L-glutamine, and 2.2 g/L sodium bicarbonate