Cell line derived from IOSE-80 (human ovarian surface epithelial cell immortalized by SV40 large T and small t antigens) by transformation with hTERT. Liu et al. (2004) Cancer Res. 64: 1655-1663.

Cell Type:
Ovary Epith.
Tissue Origin:
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:

Recommended Media

Medium 199 was developed in 1950 and was formulated for nutritional studies on chick embryo muscle fibroblasts. Its unique components include purines (adenine, guanine, xanthine, hypoxanthine), pyrimdines (thymine, uracil) and fat soluble compounds. Its current uses include virology and vaccine production.
These formulations require a CO2 atmosphere.

Storage = 2ºC to 8ºC

12-109 - with Hank's BSS, L-glutamine, and 1.4 g/L sodium bicarbonate
12-118 - with Hank's BSS, L-glutamine, HEPES, and 1.4 g/L sodium bicarbonate
12-117 - with Earle's BSS, L-glutamine, HEPES, and 2.2 g/L sodium bicarbonate
12-119 - with Earle's BSS, L-glutamine, and 2.2 g/L sodium bicarbonate

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.