H9c2(2-1)

H9c2(2-1) myoblast cell line is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle.

Cell Type:
Myoblast
Tissue Origin:
heart
Species:
rat
Research Area:
Cardiovascular
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF DS-120 1e6 90% 54% Plasmid (general) 2 µg 100 µl 4D X-Unit
SF DS-120 2e5 80% 72% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
L C-020 4e5 86% ±4 90% ±4 Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Valdés-Mas R, Gutiérrez-Fernández A, Gómez J, Coto E, Astudillo A, Puente DA, Reguero JR, Alvarez V, Morís C, León D, Martín M, Puente XS, López-Otín C. 
In:
Nat Commun. (2014) 5: 5326 
Categories:
 
Authors:
Kobashigawa LC, Xu YC, Padbury JF, Tseng YT, Yano N. 
In:
PLoS ONE (2014) 9(8): ePub