TF-1

premyeloid cell line from patient with erythroleukemia

Cell Type:
Erythroblast
Tissue Origin:
bone marrow
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF FF-100 4e5 43-63% 48-68% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF FF-100 2e6 54-68% 49-63% Plasmid (general) 2 µg 100 µl 4D X-Unit
T T-001 2e6 38% 82% ±3 Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Alexaki A, Quiterio SJ, Liu Y, Irish B, Kilareski E, Nonnemacher MR, Wigdahl B 
In:
DNA Cell Biol (2007) 26(6): 387-94 
Categories:
Transfection 
Authors:
Martinez-Moczygemba M, Huston DP, Lei JT 
In:
J Leukoc Biol (2007) 81(4): 1137-48 
Categories:
Transfection 
Authors:
Sanz C, Calasanz MJ, Andreu E, Richard C, Prosper F, Fernandez-Luna JL 
In:
Biochem J (2004) 384(Pt 2): 281-6