cells

Pupal ovary

Cell Type:

Insect

Tissue Origin:

ovarian

Species:

spodoptera frugiperda (army fall worm)

Research Area:

Cancer Research/Cell Biology

Basic Research

Cell Characteristics:

Adherent

Recommended Media

Insect-XPRESS

Insect-XPRESS™ Protein-free Insect Cell Medium is a formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21).
Cell densities in excess of 8.3 x 10^6 cells/ml can be achieved with suspension cultures of Sf9 cells using Insect-XPRESS™ Medium and an excess of oxygen. This formulation can also be used for stationary mono layer cultures and shake-flask cultures.
Insect-XPRESS™ Medium contains L-glutamine and supports superior production of recombinant proteins when using the Baculovirus Expression Vector System (BEVS).

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	CM-104	3e5	58-76%	82%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	CM-104	2e6	60-80%	76%	Plasmid (general)	2 µg	100 µl	4D X-Unit
R	I-014	2e6	58-60%	76-82%	Plasmid (general)	2 µg	100 µl	I/II/2b
R	I-014	2e6	81-83%	73-79%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations (8)

Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex

Categories:

Serum-free and Speciality Media

Authors:

Hill C and Boreikaite

In:

Mol Cell (2019) 73: 1217-1231

Pore-forming protein complexes from Pleurotus mushrooms kill western corn rootworm and Colorado potato beetle through targeting membrane ceramide phosphoethanolamine

Categories:

Serum-free and Speciality Media

Authors:

Panevska A and Hodnik V

In:

Scientific Reports (2019) 9(1): 5073

The Schlemm's canal is a VEGF-C/VEGFR-3-responsive lymphatic-like vessel

Categories:

Serum-free and Speciality Media

Authors:

Aspelund A, Tammela T, Antila S, Nurmi H, Leppänen VM, Zarkada G, Stanczuk L, Francois M, Mäkinen T, Saharinen P, Immonen I, Alitalo K

In:

J Clin Invest (2014) 124(9): 3975-86

Efficient expression of acetylcholine-binding protein from Aplysia californica in Bac-to-Bac system.

Categories:

Serum-free and Speciality Media

Authors:

Lin B1, Meng H1, Bing H1, Zhangsun D1, Luo S1.

In:

Biomed Res Int. (2014) 691480: Epub 2014 Jul 20

Biochemical and biological studies of mouse APOBEC3.

Categories:

Serum-free and Speciality Media

Authors:

Nair S1, Sanchez-Martinez S, Ji X, Rein A.

In:

J Virol (2014) 88(7): 3850-60

Enhanced expression of full-length human cytomegalovirus fusion protein in non-swelling baculovirus-infected cells with a minimal fed-batch strategy

Categories:

Serum-free and Speciality Media

Authors:

Patrone M, Carinhas N, Sousa MQ, Peixoto C, Ciferri C, Carfì A, Alves PM

In:

PLoS ONE (2014) 9(3): e90753

In Silico screening on the three-dimensional model of the Plasmodium vivax SUB1 protease leads to the validation of a novel anti-parasite compound.

Categories:

Serum-free and Speciality Media

Authors:

Bouillon A1, Giganti D, Benedet C, Gorgette O, Pêtres S, Crublet E, Girard-Blanc C, Witkowski B, Ménard D, Nilges M, Mercereau-Puijalon O, Stoven V, Barale JC.

In:

J Biol Chem (2013) 288(25): 18561-73

N-glycosylations of human a1,3-fucosyltransferase IX are required for full enzyme activity

Categories:

Serum-free and Speciality Media

Authors:

Seelhorst K1, Stacke C, Ziegelmüller P, Hahn U.

In:

Glycobiology (2013) 23(5): 559-67

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