Sf9

Pupal ovary

Cell Type:
Insect
Tissue Origin:
ovarian
Species:
spodoptera frugiperda (army fall worm)
Research Area:
Cancer Research/Cell Biology
Basic Research
Cell Characteristics:
Adherent

Recommended Media

A modification of Grace's Insect Medium developed by Drs. Gardiner and Stockdale, this medium optimizes the expression of baculovirus grown in cultured cells derived from the fall army worm, Spodoptera frugiperda. It is termed BML-TC/10, but is more commonly referred to as TC-100.

Storage = 2ºC to 8ºC 

Grace's Insect Medium was developed in 1962 and has been shown, with the addition of serum, to support the growth of insect cells, including Spodoptera frugiperda. Unsupplemented Grace's Insect Culture Medium is ideal for transfection because it does not contain lactalbumin hydrolysate, or yeastolate which can interfere with transfection.
When supplemented with L-glutamine, lactalbumin hydrolysate, and yeastolate Grace's Insect Medium can be used for general growth and maintenance of insect cells.

Storage = 2ºC to 8ºC

04-457 has no lactalbumin hydrolysate, yeastolate, gentamycin or FBS
04-649 has lactalbumin hydrolysate, yeastolate, and gentamycin, but no FBS 
04-501 Grace's Complete Insect Medium - has lactalbumin hydrolysate, yeastolate, gentamycin and 10% heat-inactivated FBS 

Insect-XPRESS™ Protein-free Insect Cell Medium is a formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21).
Cell densities in excess of 8.3 x 10^6 cells/ml can be achieved with suspension cultures of Sf9 cells using Insect-XPRESS™ Medium and an excess of oxygen. This formulation can also be used for stationary mono layer cultures and shake-flask cultures.
Insect-XPRESS™ Medium contains L-glutamine and supports superior production of recombinant proteins when using the Baculovirus Expression Vector System (BEVS).

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R I-014 2e6 58-60% 76-82% Plasmid (general) 2 µg 100 µl I/II/2b
R I-014 2e6 81-83% 73-79% Plasmid (general) 2 µg 100 µl I/II/2b
SF CM-104 3e5 58-76% 82% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF CM-104 2e6 60-80% 76% Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations

Categories:
Serum-free and Speciality Media 
Authors:
Lin B1, Meng H1, Bing H1, Zhangsun D1, Luo S1. 
In:
Biomed Res Int. (2014) 691480: Epub 2014 Jul 20