U251

glioblastoma cell line

Cell Type:
Glial Cells
Tissue Origin:
brain
Species:
human
Research Area:
Cancer Research/Cell Biology
Neurobiology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-020 1e6 63% 95% Plasmid (general) 2 µg 100 µl I/II/2b
V T-020 1e6 siRNA 3 µg 100 µl I/II/2b
SE DS-138 3e5 90% 92.3% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations

Categories:
Transfection 
Authors:
Jiang W, Cazacu S, Xiang C, Zenklusen JC, Fine HA, Berens M, Armstrong B, Brodie C, Mikkelsen T 
In:
Neoplasia (2008) 10(3): 235-43 
Categories:
Transfection 
Authors:
Kaul A, Overmeyer JH, Maltese WA 
In:
Cell Signal (2007) 19(5): 1034-43 
Categories:
Transfection 
Authors:
Katayama M, Kawaguchi T, Berger MS, Pieper RO 
In:
Cell Death Differ (2007) 14(3): 548-58 
Categories:
Transfection 
Authors:
Okhrimenko H, Lu W, Xiang C, Hamburger N, Kazimirsky G and Brodie C 
In:
Cancer Res (2005) 65(16): 7301-7309