mIMCD3
Export
kidney, medulla; collecting duct; SV40 transformed
Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
R
T-020
1e6
68%
86%
Plasmid (general)
5 µg
100 µl
I/II/2b
R
T-024
1e6
70%
87%
Plasmid (general)
5 µg
100 µl
I/II/2b
R
U-017
1e6
70%
91%
Plasmid (general)
5 µg
100 µl
I/II/2b
R
U-020
1e6
68%
88%
Plasmid (general)
5 µg
100 µl
I/II/2b
SF
FP-100
2e5
90%
80%
Plasmid (general)
400 ng
20 µl
96-well
SF
DS-137
2e5
90%
70%
Plasmid (general)
400 ng
20 µl
96-well
SF
DS-130
2e5
90%
70%
Plasmid (general)
400 ng
20 µl
96-well
Citations
Vasopressin-induced membrane trafficking of TRPC3 and AQP2 channels in cells of the rat renal collecting duct
Categories:
Transfection
Authors:
Goel M, Sinkins WG, Zuo CD, Hopfer U, Schilling WP
In:
Am J Physiol Renal Physiol (2007) 293(5): F1476-88
Collagenase-2 and -3 mediate epidermal growth factor receptor transactivation by bradykinin b2 receptor in kidney cells
Categories:
Transfection
Authors:
Mukhin YV, Gooz M, Raymond JR, Garnovskaya MN
In:
J Pharmacol Exp Ther (2006) 318(3): 1033-43