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Transfection
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What is the difference between each of the types of
HUVEC
s that Lonza offers?
When choosing between the various types of
HUVECs
that Lonza offers, the choice can be made by answering the following questions:What media do you intend to culture the cells in?Do you require pooled or single donor cells?Do you require additional...
How many donors are pooled to create the pooled human cells?
Lonza is offering the following primary cells from single donors and from pooled donors:
HUVEC
(human umbilical vein endothelial cells)NHEK-neo (human epidermal keratinocytes)HMVEC-dNeo (human microvascular endothelial cells, dermal, neonatal)When...
In my Nucleofection™ Experiment I see lower expression with my IRES construct in comparison with you pmax-GFP control. Do you have any information about the different expression profiles?
...the Nucleofector™ Technology" The data generated by our Research department show the different expression profiles of 3 vectors in HL-60 and
HUVEC
cells. You can see that our pmaxGFP cloned into three different vector sites show a different expression level....
What are differences between the endothelial cell types isolated from different locations in the body?
The group of Chi et al analayzed various endothelial cells. Tissue-specific expression patterns in different tissue microvascular endothelial cells suggest they are distinct differentiated cell types that play roles in the local physiology of...