Search Results

First siRNA library screening in hard-to-transfect HUVEC cells.

...of the Amaxa(R) Nucleofector(R) 96-well Shuttle(R) System for siRNA screening in primary cells. Lonza's Clonetics(R) HUVEC-Human Umbilical Vein Endothelial Cells were transfected with Thermo Scientific Dharmacon siGENOME(R) siRNA Libraries targeting protein...

Authors: Zumbansen M, Altrogge LM, Spottke NU, Spicker S, Offizier SM, Domzalski SB, St Amand AL, Toell A,...

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Effect of PIP3 on adhesion molecules and adhesion of THP-1 monocytes to HUVEC treated with high glucose

BACKGROUND: Phosphatidylinositol-3,4,5-triphosphate (PIP3), a well-known lipid second messenger, plays a key role in insulin signaling and glucose homeostasis. Using human umbilical vein endothelial cells (HUVEC) and THP-1 monocytes, we tested...

Authors: Manna P, Jain SK

Hyperglycemia induces differential change in oxidative stress at gene expression and functional levels in HUVEC and HMVEC.

...approach. In this study, we investigated hyperglycemia-induced endothelial dysfunction in HUVEC and HMVEC. We investigated hyperglycemia-induced functional changes (superoxide (O2?), and hydrogen peroxide (H2O2) production and mitochondrial membrane...

Authors: Patel H1, Chen J, Das KC, Kavdia M.

Hydrogen sulfide protects HUVECs against hydrogen peroxide induced mitochondrial dysfunction and oxidative stress

...levels, exerts a concentration dependent protective effect in human umbilical vein endothelial cells (HUVECs) exposed to H2O2. Analysis of ATP synthesis, mitochondrial membrane potential (??m) and cytochrome c release from mitochondria indicated...

Authors: Wen YD, Wang H, Kho SH, Rinkiko S, Sheng X, Shen HM, Zhu YZ

HIV Tat induces expression of ICAM-1 in HUVECs: implications for miR-221/-222 in HIV-associated cardiomyopathy

...protein, which is the transactivator of transcription (Tat), plays a key role in activating endothelial cells. In the present study, we demonstrated that exposure of HUVECs to HIV Tat protein resulted in induced expression of cell adhesion molecules...

Authors: Duan M, Yao H, Hu G, Chen X, Lund AK, Buch S

Plant proteolytic enzyme papain abrogates angiogenic activation of human umbilical vein endothelial cells (HUVEC) in vitro

...endothelial cells (HUVEC) in vitro. METHODS: Cell viability after prolonged treatment with papain was investigated by life cell staining and lactate dehydrogenase release assay. Angiogenic activation was assessed by ELISA against phosphorylated proteins AKT...

Authors: Mohr T, Desser L

Cellular pharmacokinetics and intracellular activity of torezolid (TR-700): studies with human macrophage (THP-1) and endothelial (HUVEC) cell lines.

...and antibacterial activity in comparison with linezolid using human macrophages (THP-1) and human endothelial cells [human umbilical vein endothelial cells (HUVECs)], applying models allowing for the quantitative evaluation of the pharmacodynamics of antibiotics...

Authors: Lemaire S, Van Bambeke F, Appelbaum PC, Tulkens PM.

Sphingosine-1-phosphate protects against bisphosphonate-induced HUVEC cell death via regulation of c-Jun-N-terminal kinase signaling

...endothelial cells (HUVECs). HUVECs were treated with S1P for 1 h and then with BP including alendronate, zoledronate and risedronate. S1P protects HUVECs against BP-induced cell death and the protective effect was increased by S1P in a dose-dependent manner...

Authors: Lee YJ, Jeong JK, Lee JH, Park YG, Moon JH, Seol JW, Jackson CJ, Park SY

Ephrin-A1 is up-regulated by hypoxia in cancer cells and promotes angiogenesis of HUVECs through a coordinated cross-talk with eNOS

...in human umbilical vein endothelial cells (HUVECs) was observed in ephrin-A1-induced angiogenesis which was reversed after co-culture with eNOS specific inhibitor, N-nitro-L-arginine methyl ester hydrochloride (L-NAME). Western blot analysis confirmed...

Authors: Song Y, Zhao XP, Song K, Shang ZJ

Formation of three-dimensional tubular endothelial cell networks under defined serum-free cell culture conditions in human collagen hydrogels.

...vein endothelial cells (HUVECs) in combination with human adipose tissue derived stromal cells (hASCs) employing human collagen I as hydrogel and decellularized porcine small intestinal submucosa as starter matrix. Matrigel/rat...

Authors: Andrée B, Ichanti H, Kalies S, Heisterkamp A, Strauß S, Vogt PM, Haverich A, Hilfiker A

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