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4535 results sorted by

High-resolution multicolor imaging of dynamic signaling complexes in T cells stimulated by planar substrates

The dynamic visualization of developing immunological synapses has been hindered by the difficulty of imaging the contact between the T cell and the antigen-presenting cell (APC). Here, we describe a technique in which T cell responses are...
Authors: Bunnell SC, Barr VA, Fuller CL and Samelson LE

GATA-3 in Human T Cell Helper Type 2 Development

The delineation of the in vivo role of GATA-3 in human T cell differentiation is a critical step in the understanding of molecular mechanisms directing human immune responses. We examined T cell differentiation and T cell-mediated effector functions...
Authors: Skapenko A, Leipe J, Niesner U, Devriendt K, Beetz R, Radbruch A, Kalden JR, Lipsky PE and...

Endothelial cells stimulate T cell NFAT nuclear translocation in the presence of cyclosporin A: involvement of the wnt/glycogen synthase kinase-3beta pathway

T cells resistant to the immunosuppressive drug cyclosporin A (CsA) may be important mediators of chronic graft rejection. We previously reported that T cells activated in the presence of endothelial cells (EC) develop resistance to CsA, and initiate...
Authors: Salazar Murphy LL and Hughes CCW

T cell receptor ligation induces the formation of dynamically regulated signaling assemblies.

Tcell antigen receptor (TCR) ligation initiates tyrosine kinase activation, signaling complex assembly, and immune synapse formation. Here, we studied the kinetics and mechanics of signaling complex formation in live Jurkat leukemic T cells using...
Authors: Bunnell SC, Hong DI, Kardon JR, Yamazaki T, McGlade CJ, Barr VA and Samelson LE

Nonmuscle Myosin IIB is involved in the guidance of fibroblast migration

Although myosin II is known to play an important role in cell migration, little is known about its specific functions. We have addressed the function of one of the isoforms of myosin II, myosin IIB, by analyzing the movement and mechanical...
Authors: Lo CM, Buxton DB, Chua GCH, Dembo M, Adelstein RS and Wang YL

CD28 engagement promotes actin polymerization through the activation of the small Rho GTPase Cdc42 in human T cells

Engagement of the costimulatory molecule CD28 is an important step in the optimal activation of T cells. Nevertheless, the specific role of CD28 in the formation of the immunological synapse and cytoskeletal changes that occur upon TCR/CD3 complex...
Authors: Salazar-Fontana LI, Barr V, Samelson LE and Bierer BE

Novel single chain cAMP sensors for receptor-induced signal propagation

Cyclic adenosine monophosphate (cAMP) is a universal second messenger of many G-protein-coupled receptors and regulates a wide variety of cellular events. cAMP exerts its effects via cAMP-dependent protein kinase (PKA), cAMP-gated ion channels and...
Authors: Nikolaev VO, Bunemann M, Hein L, Hannawacker A and Lohse MJ

Determinants of Notch-3 receptor expression and signaling in vascular smooth muscle cells. Implications in cell-cycle regulation

The Notch family of receptors and ligands plays an important role in cell fate determination, vasculogenesis, and organogenesis. Mutations of the Notch-3 receptor result in an arteriopathy that predisposes to early-onset stroke. However, the...
Authors: Campos AH, Wang W, Pollmann MJ and Gibbons GH

Expression of focal adhesion kinase in acute myeloid leukemia is associated with enhanced blast migration, increased cellularity and poor prognosis

Focal adhesion kinase (FAK) is a nonreceptor tyrosine kinase playing an important role in cell motility and survival. However, very little is known about FAK in normal and leukemic myeloid cells. In this study, FAK protein expression and mRNA were...
Authors: Recher C, Ysebaert L, Beyne-Rauzy O, Mansat-De Mas V, Ruidavets JB, Cariven P, Demur C, Payrastre B,...

HSP27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance

Smac, second mitochondria-derived activator of caspases, promotes apoptosis via activation of caspases. Heat shock protein 27 (Hsp27) negatively regulates another mitochondrial protein, cytochrome c, during apoptosis; however, the role of Hsp27 in...
Authors: Chauhan D, Li G, Hideshima T, Podar K, Mitsiades C, Midsiades N, Catley L, Tai YT, Hayashi T,...
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