Q:

How do I cryopreserve Lonza’s primary cells? What media cocktail are Lonza’s primary cells cryopreserved in?

A:

Please be aware that cryopreservation may compromise cell quality and performance.

Lonza CANNOT guarantee performance of Clonetics™ & Poietics™ Cells that have been cryopreserved outside of Lonza. To avoid loss of cells and forfeiture of your warranty, we recommend keeping cells in continuous culture without cryopreservation.
For customers that still want to cryopreserve those cells on their own risk, we have a guideline.The document below details the suggested cryopreservation protocol and media for most of our primary cell types. These are the same protocols and cryopreservation media that we use for these cells when cryopreserving the cells prior to sale.

However, as mentioned above, we do not guarantee for survival and performance of the cells in this case.

Categories:
Primary Cells and Media
Research Areas:
Cancer Research/Cell Biology
Basic Research