Q:

Are there positive controls for the MycoAlert® Assay?

A:

The MycoAlert® Assay Control Set (LT07-518) is available separately and includes a lyophilized positive control (1 ml) and assay buffer (2 ml) for reconstitution.

The assay buffer also serves as a negative control. The positive and negative controls are designed for use with the MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit for the detection of mycoplasma contaminations in cell cultures. The MycoAlert® Assay Control Set may be used to ensure that the operator and all of the assay reagents are performing properly. The positive control is proprietary, but does NOT contain mycoplasma. The positive or negative control is simply substituted for a sample in the standard assay protocol. The positive control will give a ratio of >1 while the negative control will give a ratio <1.

Categories:
Mycoplasma Detection and Prevention
Research Areas:
Basic Research