Q:

I want to stimulate Human T cells post Nucleofection®. Are there any precautions to keep in mind?
A:

After Nucleofection®, wait at least four hours before stimulation. Stimulating immediately after Nucleofection® may lead to increased cell mortality and poor activation of cells. For more details on the stimulation of Human T cells post Nucleofection®, please see the Optimized Protocol.
Related Cells:
T cell, human peripheral blood unstim. ; T cell, human stim.
Categories:
Transfection
Research Areas:
Immunotherapy / Hematology

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