YY1 transcription factor down-regulates expression of CCR5, a major co-receptor for HIV-1

Authors:
Moriuchi M and Moriuchi H
In:
J Biol Chem (2003) 278(15): 13003-13007
Cells used in publication:
PBMC, human
Species: human
Tissue Origin: blood
Experiment
Expression of CCR5, a major coreceptor for HIV-1 is regulated by a number of transcription factors. This study aimed at elucidating the role of transcription factor YY1 on CCR5 expression in primary human T cells. Cells were nucleofected with an expression plasmid encoding a GFP-YY1 fusion protein. Cell surface expression of CCR5 was reduced compared to mock transfected cells. In a similar experiment nucleofected cells were infected with RS-HIV-1. Overexpression of YY1 decreased infectibility by R5-HIV-1. Both results indicate a critical role of YY1 in the pathogenesis of HIV-1.
Abstract
Expression of CCR5, a major coreceptor for human immunodeficiency virus type 1 (HIV-1), is regulated by a number of transcription factors. Here we report that the YY1 transcription factor down-regulates CCR5 promoter activity and that overexpression of YY1 reduces cell surface CCR5 expression and infectibility by R5-HIV-1. Because YY1 also down-regulates promoter activities of CXCR4, another major coreceptor for HIV-1 and HIV-1 long terminal repeat, this transcription factor may play a critical role in the pathogenesis of HIV-1 disease.