cells

Human leukemia cell line established from the peripheral blood of a patient at relapse of acute monocytic leukemia, FAB M5a, which had evolved from myelodysplastic syndrome (MDS). Matsuo et al. (1997) Leukemia 11(9):1469-77.

Cell Type:

Monocyte

Tissue Origin:

blood

Species:

human

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	C	X-001	4e6	50%	80%	Plasmid (general)	2 µg	100 µl	I/II/2b
	C	X-005	4e6	55%	75%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

Block of C/EBP function by phosphorylation in acute myeloid leukemia with FLT3 activating mutations

Categories:

Transfection

Authors:

Radomska HS, Basseres DS, Zheng R, Zhang P, Dayaram T, Yamamoto Y, Sternberg DW, Lokker N, Giese NA, Bohlander SK, Schnittger S, Delmotte MH, Davis RJ, Small D, Hiddemann W, Gilliland DG and Tenen DG

In:

J Exp Med (2006) 203(2): 371-81

MOLM-14

Transfection Information

Citations