A-375
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Malignant melanoma skin. Also called A375.
Cell Type:
Skin/Hair Epith.
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
FF-120
2e5
92% ±3
95%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SF
FF-120
1e6
93% ±2
83%
Plasmid (general)
2 µg
100 µl
4D X-Unit
V
X-001
1e6
72% ±2
97% ±1
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Recombinant interleukin-24 lacks apoptosis-inducing properties in melanoma cells
Categories:
Transfection
Authors:
Kreis S, Philippidou D, Margue C, Rolvering C, Haan C, Dumoutier L, Renauld JC, Behrmann I
In:
PLoS ONE (2007) 2(12): e1300
Ras-associated protein-1 regulates extracellular signal-regulated kinase activation and migration in melanoma cells: two processes important to melanoma tumorigenesis and metastasis
Categories:
Transfection
Authors:
Gao L, Feng Y, Bowers R, Becker-Hapak M, Gardner J, Council L, Linette G, Zhao H, Cornelius LA
In:
Cancer Res (2006) 66(16): 7880-8
Targeting Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Kinase in the Mutant (V600E) B-Raf Signaling Cascade Effectively Inhibits Melanoma Lung Metastases
Categories:
Transfection
Authors:
Sharma A, Tran MA, Liang S, Sharma AK, Amin S, Smith CD, Dong C, Robertson GP
In:
Cancer Res (2006) 66(16): 8200-9