MEG-01
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Megakaryoblast from Chronic myelogenous leukemia (CML)
Cell Type:
Megakaryocyte
Tissue Origin:
bone marrow
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
C
X-005
1e6
80% ±4
66% ±2
Plasmid (general)
2 µg
100 µl
I/II/2b
SE
EN-102
1e6
74% ±1
79% ±17
Plasmid (general)
2 µg
100 µl
4D X-Unit
SE
EN-102
2e5
67% ±1
61% ±4
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
Citations
Fusion of One twenty-two to BSAC (Basic, SAP, and coiled-coil domain) results in aberrant upregulation of transcriptional activity
Categories:
Transfection
Authors:
Sawada T, Nishiyama C, Kishi T, Sasazuki T, Komazawa-Sakon S, Xue X, Piao JH, Ogata H, Nakayama JI, Taki T, Hayashi Y, Watanabe M, Yagita H, Okumura K, Nakano H
In:
J Biol Chem (2008) epub: online
An LRP8 variant is associated with familial and premature coronary artery disease and myocardial infarction
Categories:
Transfection
Authors:
Shen GQ, Li L, Girelli D, Seidelmann SB, Rao S, Fan C, Park JE, Xi Q, Li J, Hu Y, Olivieri O, Marchant K, Barnard J, Corrocher R, Elston R, Cassano J, Henderson S, Hazen SL, Plow EF, Topol EJ, Wang QK
In:
Am J Hum Genet (2007) 81(4): 780-91