Hepatocyte, human

Human hepatocytes (either freshly isolated or cryopreserved)

Cell Type:
Liver Epith.
Tissue Origin:
liver
Species:
human
Research Area:
Cancer Research/Cell Biology
Toxicology
Cell Characteristics:
Adherent

Recommended Media

In 1971, Williams et al. described a procedure for enriching isolated hepatocyte cultures for polygonal epithelial cells and reducing the number of contaminating fibroblasts. This method was a sequential plating technique based on the observation that fibroblast-like cells adhere to a substrate more rapidly than epithelial cells. The isolated epithelial cells resulting from this treatment could then be cultured in a rich medium designated Williams' Medium D. Newborn animals were the source of cells used in these studies, as they were in most studies of liver cell culture. Since newborn liver is not functionally mature, further studies were conducted by Williams and Gunn to explore the possibility of culturing adult liver on a long-term basis. The medium developed during the course of these studies was designated Williams' Medium E. It has been shown to support the growth in long-term culture of adult liver epithelial cells.

Storage = 2ºC to 8ºC

12-761 - without L glutamine
02-019 - without L-glutamine or phenol red

HCM is  a serum-free medium for maintenance of cultures prior to and during experimental procedures.
It is the preferred medium when working with cryopreserved human and animal hepatocytes.

HMM is a serum-free medium that may be used to maintain fresh plated human and animal hepatocytes in culture during testing.

Hepatic media for the plating of fresh and cryopreserved, human and animal, primary hepatocytes. The medium consists of a basal Medium with a supplement.

MP100: Hepatocyte Plating Medium w/Supplement (MP100-1 + MP100-2), 100ml
MP250: : Hepatocyte Plating Medium w/Supplement (MP250-1 + MP250-2), 250 ml

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 96-EX-147 1e5 54% 69% Plasmid (general) 1 µg 20 µl Shuttle
P3 96-EX-147 1e5 54% 59% Plasmid (general) 1 µg 20 µl Shuttle
P3

EX-147

1e5 54% 69% Plasmid (general) 1 µg 20 µl 4D X-Unit

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