Epithelial, retinal pigment (RPE), human

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The outermost layer of the retina, consisting of cuboidal epithelial cells derived from the outer layer of the embryonic optic cup. The dense melanin pigment of this layer absorbs light not captured by photoreceptors.

Cell Type:
Eye Epith.
Tissue Origin:
eye
Species:
human
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Research Area:
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

Retinal Pigment Epithelial Cell Growth Media

RtEGM is optimized and performance tested for growing Clonetics Human RPE cells. The medium does contain 2% serum

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Epithelial, basic U-017 30-40% 2 µg 100 µl I/II/2b
P3 EA-104 2e5 94.3% ±4 84.6% ±6 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 EA-104 1e6 93.7% ±2 89.2% ±14 Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations

From confluent human iPS cells to self-forming neural retina and retinal pigmented epithelium
Categories:
Transfection 
Authors:
Reichman S, Terray A, Slembrouck A, Nanteau C, Orieux G, Habeler W, Nandrot EF, Sahel JA, Monville C, Goureau O 
In:
Proc Natl Acad Sci USA (2014) 111(23): 8518-23 
A reactive oxygen species-mediated, self-perpetuating loop persistently activates platelet-derived growth factor receptor a.
Categories:
Primary Cells and Media 
Authors:
Lei H1, Kazlauskas A 
In:
Mol Cell Biol (2014) 34(1): 110-122 
Pigment epithelium-derived factor secreted from retinal pigment epithelium facilitates apoptotic cell death of iPSC
Categories:
Primary Cells and Media 
Authors:
Kanemura H1, Go MJ, Nishishita N, Sakai N, Kamao H, Sato Y, Takahashi M, Kawamata S 
In:
Scientific Reports (2013) 3:2334: 10.1038 
Cancer translocations in human cells induced by zinc finger and TALE nucleases.
Categories:
Transfection 
Authors:
Piganeau M, Ghezraoui H, De Cian A, Guittat L, Tomishima M, Perrouault L, René O, Katibah GE, Zhang L, Holmes MC, Doyon Y, Concordet JP, Giovannangeli C, Jasin M, Brunet E. 
In:
Genome Res (2013) 23(7): 1182-93 
Generation of retinal pigment epithelial cells from small molecules and OCT4 reprogrammed human induced pluripotent stem cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ukrohne TU1, Westenskow PD, Kurihara T, Friedlander DF, Lehmann M, Dorsey AL, Li W, Zhu S, Schultz A, Wang J, Siuzdak G, Ding S, Friedlander M 
In:
Other (2012) 1(2): 96-109 

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