cells

subcutaneous connective tissue; areola and adipose subcutaneous connective tissue fibroblasts, from the normal areolar and adipose tissue of C3H/An mice. Cell Line also called L-929. Derived from the parent L strain in 1948.

Cell Type:

Adipocyte

Tissue Origin:

Connective tissue proper

Species:

mouse

Research Area:

Cancer Research/Cell Biology

Dermatology/Tissue Engineering

Cell Characteristics:

Adherent

Recommended Media

NCTC-109

NCTC-109 was developed in 1956 as a protein free medium for L-929 mouse cells. Its unique components include Tween® 80, nucleic acid derivations, additional vitamins, co-enzymes (DPN, CoA, TPN, FAD, UTP, TPP) and reducing agents such as glutathione, ascorbic acid and L-cystine. It is well suited for the growth of hybridoma cell lines.

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
V	X-005	1e6	67% ±2	91% ±3	Plasmid (general)	2 µg	100 µl	I/II/2b
SE	FF-120	2e5	99.2% ±	65.8% ±	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SE	FF-120	1e6	99.2% ±	48.3% ±2	Plasmid (general)	2 µg	100 µl	4D X-Unit

Citations

MIP-1 (CCL3) is a Downstream Target of FGFR3 and RAS/MAPK Signaling in Multiple Myeloma

Categories:

Transfection

Authors:

Masih-Khan E, Trudel S, Heise C, Li Z, Paterson J, Nadeem V, Wei E, Roodman D, Claudio J, Bergsagel L, Stewart AK

In:

Blood (2006) 108(10): 3465-71

Autophagic programmed cell death by selective catalase degradation

Categories:

Transfection

Authors:

Yu L, Wan F, Dutta S, Welsh S, Liu Z, Freundt E, Baehrecke EH, Lenardo M

In:

Proc Natl Acad Sci USA (2006) 103(13): 4952-7

Ceramide mediates caspase-independent programmed cell death

Categories:

Transfection

Authors:

Thon L, Mohlig H, Mathieu S, Lange A, Bulanova E, Winoto-Morbach S, Schutze S, Bulfone-Paus S and Adam D

In:

FASEB J (2005) 19(14): 1945-1956

NCTC clone 929

Recommended Media

Transfection Information

Citations