cells

Skeletal muscle myoblast

Cell Type:

Myoblast

Tissue Origin:

skeletal muscle

Species:

rat

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SE	CD-112	2e5	56% ±8	60% ±4	Plasmid (general)	0.6 µg	20 µl	4D X-Unit
SE	CD-112	1e6	47% ±5	67% ±4	Plasmid (general)	3 µg	100 µl	4D X-Unit
R	X-005	1e6	59% ±9	92% ±2	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

Improving type 2 diabetes through a distinct adrenergic signaling pathway involving mTORC2 that mediates glucose uptake in skeletal muscle.

Categories:

Primary Cells and Media, Transfection

Authors:

Sato M, Dehvari N, Oberg AI, Dallner OS, Sandström AL, Olsen JM, Csikasz RI, Summers RJ, Hutchinson DS, Bengtsson T

In:

Diabetes (2014) Epub: ahead

Dexamethasone represses signaling through the mammalian target of rapamycin in muscle cells by enhancing expression of REDD1

Categories:

Transfection

Authors:

Wang H, Kubica N, Ellisen LW, Jefferson LS, Kimball SR

In:

J Biol Chem (2006) 281(51): 39128-34

L6

Transfection Information

Citations