HEPM

Osteoblast precursor cell, fibroblast,

Cell Type:
Bone
Tissue Origin:
Species:
human
Research Area:
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Fibroblast, basic A-024 5e5 60% 58% Plasmid (general) 2 µg 100 µl I/II/2b
Fibroblast, basic T-016 5e5 60% 54% Plasmid (general) 2 µg 100 µl I/II/2b
Fibroblast, basic U-012 5e5 70% 55% Plasmid (general) 2 µg 100 µl I/II/2b
Fibroblast, basic U-023 5e5 80% 55% Plasmid (general) 2 µg 100 µl I/II/2b
Fibroblast, basic V-013 5e5 90% 70% Plasmid (general) 2 µg 100 µl I/II/2b