DO11.10

T hybridoma cell line

Cell Type:
T Cell
Tissue Origin:
Species:
mouse
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

UltraDOMA-PF Protein-free Hydridoma Media are formulations designed for use with hybridoma cell lines of murine, human, and chimeric origin. UltraDOMA-PF Media are completely defined media and do not contain peptides or tissue extracts.
The use of UltraDOMA-PF Media significantly simplifies downstream processing since all proteins present in a given cell culture supernatant are produced by the cells. 

L-glutamine and HEPES buffer are included in the formulation.

Storage = 2ºC  to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V A-020 1e6 24% 90% Plasmid (general) 2 µg 100 µl I/II/2b
V X-001 1e6 59% 46% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Liu WH and Lai MZ 
In:
Mol Cell Biol (2005) 25(4): 1367-1378