SMC, bladder (BdSMC), human

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Human bladder smooth muscle

Cell Type:
Smooth muscle cell
Tissue Origin:
bladder
Species:
human
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Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent

Recommended Media

Smooth Muscle Growth Medium-2

 SmGM-2 was developed for the maintenance and expansion of human smooth muscle cells. This medium contains 5% FBS.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Smooth muscle, basic D-033 2e6 80% 90% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Modeling retinitis pigmentosa through patient-derived retinal organoids
Categories:
Transfection 
Authors:
Yan-Ping Li , Wen-Li Deng , Zi-Bing Jin  
In:
STAR Protoc (2021) 2(2): 100438 
Evaluation of silk biomaterials in combination with extracellular matrix coatings for bladder tissue engineering with primary and pluripotent cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Franck D1, Gil ES, Adam RM, Kaplan DL, Chung YG, Estrada CR Jr, Mauney JR. 
In:
PLoS ONE (2013) 8(2): e56237 
Signal transducer and activator of transcription 3 activation is associated with bladder cancer cell growth and survival
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chen CL, Cen L, Kohout J, Hutzen B, Chan C, Hsieh FC, Loy A, Huang V, Cheng G, Lin J 
In:
Mol Cancer (2008) 7: 78: 1-12 

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