PANC-1

epitheloid duct carcinoma

Cell Type:
Pancreas Epith.
Tissue Origin:
pancreas
Species:
human
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE DN-100 1e6 91% 50-52% Plasmid (general) 2 µg 100 µl 4D X-Unit
SE DN-100 2e5 82-84% 54-68% Plasmid (general) 400 ng 20 µl 4D X-Unit
R X-005 1e6 67-69% 71-79% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Live Cell Imaging 
Authors:
Brunetti J, Depau L, Falciani C, Gentile M, Mandarini E, Riolo G, Lupetti P, Pini A, Bracci L 
In:
Scientific Reports (2016) 6: 27174 
Categories:
Transfection 
Authors:
Edderkaoui M, Hong P, Lee JK, Pandol SJ, Gukovskaya AS 
In:
J Biol Chem (2007) 282(37): 26646-55 
Categories:
Transfection 
Authors:
Ohuchida K, Mizumoto K, Ogura Y, Ishikawa N, Nagai E, Yamaguchi K and Tanaka M 
In:
Clin Cancer Res (2005) 11(6): 2285-2292