SW480

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adenocarcinoma
Cell Type:
GI Tract Epith.
Tissue Origin:
colon
Species:
human
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent

Recommended Media

L-15 (Leibovitz)

L-15 (Leibovitz) Medium was developed (1963) for fast growing tumor cells and was formulated for use without CO2 enriched atmosphere. Its unique features include: free base forms of arginine, cysteine and histidine; high concentration of tyrosine; galactose used instead of glucose and no sodium bicarbonate.
L-15 is used to grow cell lines in the absence of CO2 enriched atmosphere.  The bicarbonate-free medium is buffered with elevated levels of amino acids.

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V L-024 1e6 58-62% 85-87% Plasmid (general) 2 µg 100 µl I/II/2b
SF DN-100 2e5 68-76% 76-94% Plasmid (general) 0.8 µg 20 µl 4D X-Unit
SF DN-100 1e6 56-60% 65-71% Plasmid (general) 4 µg 100 µl 4D X-Unit

Citations (7)

Vascular endothelial growth factor receptor-1 contributes to resistance to anti-epidermal growth factor receptor drugs in human cancer cells
Categories:
Transfection 
Authors:
Bianco R, Rosa R, Damiano V, Daniele G, Gelardi T, Garofalo S, Tarallo V, De Falco S, Melisi D, Benelli R, Albini A, Ryan A, Ciardiello F, Tortora G 
In:
Clin Cancer Res (2008) 14(16): 5069-80 
BUB1 mediation of caspase-independent mitotic death determines cell fate
Categories:
Transfection 
Authors:
Niikura Y, Dixit A, Scott R, Perkins G, Kitagawa K 
In:
J Cell Biol (2007) 178(2): 283-96 
Fascin, a novel target of beta-catenin-TCF signaling, is expressed at the invasive front of human colon cancer
Categories:
Transfection 
Authors:
Vignjevic D, Schoumacher M, Gavert N, Janssen KP, Jih G, Laé M, Louvard D, Ben-Ze'ev A, Robine S 
In:
Cancer Res (2007) 67(14): 6844-53 
Protein phosphatase 2A is required for mesalazine-dependent inhibition of Wnt/-catenin pathway activity
Categories:
Transfection 
Authors:
Bos CL, Diks SH, Hardwick JC, Walburg KV, Peppelenbosch MP, Richel DJ 
In:
Carcinogenesis (2006) 27(12): 2371-82 
The histidine triad protein Hint1 triggers apoptosis independent of its enzymatic activity
Categories:
Transfection 
Authors:
Weiske J, Huber O 
In:
J Biol Chem (2006) 281(37): 27356-66 
Gli1, down-regulated in colorectal cancers, inhibits proliferation of colon cancer cells involving Wnt signalling activation
Categories:
Transfection 
Authors:
Akiyoshi T, Nakamura M, Koga K, Nakashima H, Yao T, Tsuneyoshi M, Tanaka M and Katano M 
In:
Gut (2006) 55(7): 991-9 
Elevated expression of C10orf3 (chromosome 10 open reading frame 3) is involved in the growth of human colon tumor
Categories:
Transfection 
Authors:
Sakai M, Shimokawa T, Kobayashi T, Matsushima S, Yamada Y, Nakamura Y and Furukawa Y 
In:
Oncogene (2006) 25(3): 480-6 
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