cells

Breast adenocarcinoma cells

Cell Type:

Mammary Epith.

Tissue Origin:

breast

Species:

human

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Recommended Media

L-15 (Leibovitz)

L-15 (Leibovitz) Medium was developed (1963) for fast growing tumor cells and was formulated for use without CO2 enriched atmosphere. Its unique features include: free base forms of arginine, cysteine and histidine; high concentration of tyrosine; galactose used instead of glucose and no sodium bicarbonate.
L-15 is used to grow cell lines in the absence of CO2 enriched atmosphere. The bicarbonate-free medium is buffered with elevated levels of amino acids.

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SE	CH-125	4e5	72% ±1	64% ±7	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SE	CH-125	2e6	75% ±1	67% ±3	Plasmid (general)	2 µg	100 µl	4D X-Unit
V	X-013	1e6	77-81%	76-78%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations (18)

6-Shogaol Inhibits Breast Cancer Cells and Stem Cell-Like Spheroids by Modulation of Notch Signaling Pathway and Induction of Autophagic Cell Death

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Ray A, Vasudevan S, Sengupta S

In:

PLoS ONE (2015) 10(9): e0137614

Novel inhibitors induce large conformational changes of GAB1 pleckstrin homology domain and kill breast cancer cells

Categories:

Primary Cells and Media

Authors:

Chen L, Du-Cuny L, Moses S, Dumas S, Song Z, Rezaeian AH, Lin HK, Meuillet EJ, Zhang S

In:

Other (2015) 11(1): e1004021

A p120 catenin isoform switch affects Rho activity, induces tumor cell invasion and predicts metastatic disease

Categories:

Transfection

Authors:

Yanagisawa M, Huveldt D, Kreinest P, Lohse CM, Cheville JC, Parker AS, Copland JA, Anastasiadis PZ

In:

J Biol Chem (2008) 283(26): 18344-54

Increased susceptibility of breast cancer cells to stress mediated inhibition of protein synthesis

Categories:

Transfection

Authors:

Pervin S, Tran AH, Zekavati S, Fukuto JM, Singh R, Chaudhuri G

In:

Cancer Res (2008) 68(12): 4862-74

Selective inhibition of histone deacetylase 2 silences progesterone receptor-mediated signaling

Categories:

Transfection

Authors:

Bicaku E, Marchion DC, Schmitt ML, Münster PN

In:

Cancer Res (2008) 68(5): 1513-9

The Nuclear Matrix Protein, NRP/B, Enhances Nrf2-Mediated Oxidative Stress Responses in Breast Cancer Cells

Categories:

Transfection

Authors:

Seng S, Avraham HK, Jiang S, Yang S, Sekine M, Kimelman N, Li H, Avraham S

In:

Cancer Res (2007) 67(18): 8596-604

Modulation of estrogen receptor alpha protein level and survival function by DBC-1

Categories:

Transfection

Authors:

Trauernicht AM, Kim SJ, Kim NH, Boyer TG

In:

Mol Endocrinol (2007) 21(7): 1526-36

Easy stable transfection of a human cancer cell line by electrogene transfer with an Epstein-Barr virus-based plasmid vector

Categories:

Transfection

Authors:

Shibata MA, Miwa Y, Morimoto J, Otsuki Y

In:

Med Mol Morphol (2007) 40(2): 103-7

Loss of fibulin-2 expression is associated with breast cancer progression

Categories:

Transfection

Authors:

Yi CH, Smith DJ, West WW, Hollingsworth MA

In:

Am J Pathol (2007) 170(5): 1535-45

Nitric oxide in physiologic concentrations targets the translational machinery to increase the proliferation of human breast cancer cells: involvement of mammalian target of rapamycin/eIF4E pathway

Categories:

Transfection

Authors:

Pervin S, Singh R, Hernandez E, Wu G, Chaudhuri G

In:

Cancer Res (2007) 67(1): 289-99

A cancer cell metalloprotease triad regulates the basement membrane transmigration program

Categories:

Transfection

Authors:

Hotary K, Li XY, Allen E, Stevens SL, Weiss SJ

In:

Genes Dev (2006) 20(19): 2673-86

ADAM28 Is Overexpressed in Human Breast Carcinomas: Implications for Carcinoma Cell Proliferation through Cleavage of Insulin-like Growth Factor Binding Protein-3

Categories:

Transfection

Authors:

Mitsui Y, Mochizuki S, Kodama T, Shimoda M, Ohtsuka T, Shiomi T, Chijiiwa M, Ikeda T, Kitajima M, Okada Y

In:

Cancer Res (2006) 66(20): 9913-20

Noninvasive optical tracking of red fluorescent protein-expressing cancer cells in a model of metastatic breast cancer

Categories:

Transfection

Authors:

Winnard PT Jr, Kluth JB, Raman V

In:

Neoplasia (2006) 8(10): 796-806

MEKK1 controls matrix degradation and tumor cell dissemination during metastasis of polyoma middle-T driven mammary cancer

Categories:

Transfection

Authors:

Cuevas BD, Winter-Vann AM, Johnson NL, Johnson GL

In:

Oncogene (2006) 25(36): 4998-5010

Distinct Roles of Nonmuscle Myosin II Isoforms in the Regulation of MDA-MB-231 Breast Cancer Cell Spreading and Migration

Categories:

Transfection

Authors:

Betapudi V, Licate LS, Egelhoff TT

In:

Cancer Res (2006) 66(9): 4725-33

Protease-Activated Receptor-2 Is Essential for Factor VIIa and Xa-Induced Signaling, Migration, and Invasion of Breast Cancer Cells

Categories:

Transfection

Authors:

Morris DR, Ding Y, Ricks TK, Gullapalli A, Wolfe BL and Trejo J

In:

Cancer Res (2006) 66(1): 307-314

Electrogene transfer of an Epstein-Barr virus-based plasmid replicon vector containing the diphtheria toxin A gene suppresses mammary carcinoma growth in SCID mice

Categories:

Transfection

Authors:

Shibata MA, Miwa Y, Miyashita M, Morimoto J, Abe H and Otsuki Y

In:

Cancer Sci (2005) 96(7): 434-440

Extracellular Nef protein targets CD4+ T cells for apoptosis by interacting with CXCR4 surface receptors.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

James CO, Huang MB, Khan M, Garcia-Barrio M, Powell MD, Bond VC

In:

J Virol (2004) 78(6): 3099-109

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