A-431

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epidermoid carcinoma

Cell Type:
Uterine/Vaginal Epith.
Tissue Origin:
uterus
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF EQ-100 4e5 40-52% 67-71% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF EQ-100 2e6 45-57% 70-74% Plasmid (general) 2 µg 100 µl 4D X-Unit
T X-001 1e6 43-47% 79-87% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Inhibition of Phosphatidylcholine-Specific Phospholipase C Interferes with Proliferation and Survival of Tumor Initiating Cells in Squamous Cell Carcinoma
Categories:
Primary Cells and Media, Classical Media and Reagents 
Authors:
Cecchetti S, Bortolomai I, Ferri R, Mercurio L, Canevari S, Podo F, Miotti S, Iorio E. 
In:
PLoS ONE (2015) 10(9): e0136120 
Direct Repression of Cyclin D1 by SIP1 Attenuates Cell Cycle Progression in Cells Undergoing an Epithelial Mesenchymal Transition
Categories:
Transfection 
Authors:
Mejlvang J, Kriajevska M, Vandewalle C, Chernova T, Sayan AE, Berx G, Mellon JK, Tulchinsky E 
In:
Mol Biol Cell (2007) 18(11): 4615-4624 
Construction of a Three-Dimensional Human Skin Model Consisting of Keratinocytes, Dendritic Cells and Fibroblasts and Application of This Model for Alternative Animal Testing of Immune-Sensitizing Compounds
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Uchino T, Ikarashi Y, Tokunaga H 
In:
Other (2007) 41(4): 246-253 
Distinct Roles for Tsg101 and Hrs in Multivesicular Body Formation and Inward Vesiculation
Categories:
Transfection 
Authors:
Razi M, Futter CE 
In:
Mol Biol Cell (2006) 17(8): 3469-83 

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