HuT 78

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cutaneous T cell lymphoma

Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

Iscove's Modified Dulbecco's Medium

Iscove's Modified Dulbecco's Medium (IMDM) is a modified DME containing high glucose (4,500 mg/L), sodium pyruvate, additional amino acids, HEPES buffer (in liquid form), selenium and other components. IMDM is suitable for fast growing cells and can support the growth of a wide variety of mammalian cells.

Storage = 2ºC to 8ºC

12-722 with L-glutamine
12-726 without L-glutamine
12-915 with L-glutamine and screened to support hybridoma growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF DF-110 2e5 48-50% 53-65% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF DF-110 1e6 45-47% 60-68% Plasmid (general) 2 µg 100 µl 4D X-Unit
R V-001 2e6 49-57% 53-75% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Arrestin 3 mediates endocytosis of CCR7 following ligation of CCL19 but not CCL21
Categories:
Transfection 
Authors:
Byers MA, Calloway PA, Shannon L, Cunningham HD, Smith S, Li F, Fassold BC, Vines CM 
In:
J Immunol (2008) 181(7): 4723-32 
CREB, ATF, and AP-1 transcription factors regulate IFN-gamma secretion by human T cells in response to mycobacterial antigen
Categories:
Transfection 
Authors:
Samten B, Townsend JC, Weis SE, Bhoumik A, Klucar P, Shams H, Barnes PF 
In:
J Immunol (2008) 181(3): 2056-64 
Epigenetic mechanisms of age-dependent KIR2DL4 expression in T cells
Categories:
Transfection 
Authors:
Li G, Weyand CM, Goronzy JJ 
In:
J Leukoc Biol (2008) epub: online 
The cytosolic protein talin induces an intermediate affinity integrin alphaLbeta2
Categories:
Transfection 
Authors:
Li YF, Tang RH, Puan KJ, Law SK, Tan SM 
In:
J Biol Chem (2007) 282(33): 24310-9 
Inhibition of BCL11B expression leads to apoptosis of malignant but not normal mature T cells
Categories:
Transfection 
Authors:
Grabarczyk P, Przybylski GK, Depke M, Volker U, Bahr J, Assmus K, Broker BM, Walther R, Schmidt CA 
In:
Oncogene (2007) 26(26): 3797-810 

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