MDCK II

Madin-Darby Canine Cocker Spaniel kidney cell, Polarised epithelial cells, MDCK II cells were obtained from higher passage MDCK cells

Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
canine
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

UltraMDCK Serum-free Renal Cell Medium is designed to support the growth of MADIN-DARBY Canine Kidney (MDCK) cells at low and high plating densities.
The medium contains only two proteins: recombinant human insulin and bovine transferrin, yielding a very low protein formulation.

Storage = 2ºC to 8ºC

ProVero™ 1 Serum-free Medium is a protein-free medium designed to support the growth of MDCK and vero cells. It contains HEPES and sodium bicarbonate buffer.
The absence of proteins and only very low levels of human recombinant insulin facilitate both downstream processing and regulatory compliance.

Some Vero cell strains require additional supplementation with 5.0 µg/L rhEGF for optimal vero cell growth.

Storage = 2°C to 8°C

ProMDCK™ 2D is a non-animal-origin (NAO) serum free medium that supports the growth of Madin-Darby Canine Kidney Cells (MDCK) in cell culture.  ProMDCK™ 2D medium is optimized for expansion and virus infection of MDCK cells in planar culture (2D)

ProMDCK™ 3D is designed for growth of MDCK cells in 3D culture on microcarrier. ProMDCK™ 3D medium allows direct transition of MDCK cells from ProMDCK™ 2D cultures onto microcarriers

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
L L-005 1e6 76-84% 84-92% Plasmid (general) 2 µg 100 µl I/II/2b
SF DY-100 2e5 86.9-90.9% 61.4-71.4% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF DY-100 1e6 91.7-93.7% 61.6-75.6% Plasmid (general) 2 µg 100 µl 4D X-Unit
SE CM-113 1e5 96-98% 97-100% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE CM-113 1e6 94% 100% Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations (8)

Categories:
Transfection 
Authors:
Friedrichs J, Torkko JM, Helenius J, Teräväinen TP, Füllekrug J, Muller DJ, Simons K, Manninen A 
In:
J Biol Chem (2007) 282(40): 29375-83 
Categories:
Transfection 
Authors:
Gravotta D, Deora A, Perret E, Oyanadel C, Soza A, Schreiner R, Gonzalez A, Rodriguez-Boulan E 
In:
Proc Natl Acad Sci USA (2007) 104(5): 1564-9 
Categories:
Transfection 
Authors:
Hoppe S, Schelhaas M, Jaeger V, Liebig T, Petermann P, Knebel-Morsdorf D 
In:
J Gen Virol (2006) 87(Pt 12): 3483-94 
Categories:
Transfection 
Authors:
Qin Y, Capaldo C, Gumbiner BM and Macara IG 
In:
J Cell Biol (2005) 171(6): 1061-1071 
Categories:
Transfection 
Authors:
Deora AA, Philp N, Hu J, Bok D and Rodriguez-Boulan E 
In:
Proc Natl Acad Sci USA (2005) 102(45): 16245-16250 
Categories:
Transfection 
Authors:
Meder D, Shevchenko A, Simons K and Fullekrug J 
In:
J Cell Biol (2005) 168(2): 303-313 
Categories:
Transfection 
Authors:
Du Q and Macara IG 
In:
Cell (2004) 119(4): 503-516 
Categories:
Transfection 
Authors:
Gao L and Macara IG 
In:
J Biol Chem (2004) 279(40): 41557-41562 
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