MOLT-4
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acute lymphoblastic leukemia
Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
L
C-005
2e6
50-60%
59-63%
Plasmid (general)
2 µg
100 µl
I/II/2b
SF
CA-137
2e5
76-78%
65-83%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SF
CA-137
1e6
81-83%
55-79%
Plasmid (general)
2 µg
100 µl
4D X-Unit
Citations (8)
Overexpression of mitochondrial methionine sulfoxide reductase B2 protects Leukemia cells from oxidative stress-induced cell death and protein damage
Categories:
Transfection
Authors:
Cabreiro F, Picot CR, Perichon M, Castel J, Friguet B, Petropoulos I
In:
J Biol Chem (2008) 283(24): 16673-16681
Epigenetic inactivation of the Groucho homologue gene TLE1 in hematologic malignancies
Categories:
Transfection
Authors:
Fraga MF, Berdasco M, Ballestar E, Ropero S, Lopez-Nieva P, Lopez-Serra L, MartÜn-Subero JI, Calasanz MJ, de Silanes IL, Setien F, Casado S, Fernandez AF, Siebert R, Stifani S, Esteller M
In:
Cancer Res (2008) 68(11): 4116-22
Expression of SMARCB1 modulates steroid sensitivity in human lymphoblastoid cells: identification of a promoter snp that alters PARP1 binding and SMARCB1 expression
Categories:
Transfection
Authors:
Pottier N, Cheok MH, Yang W, Assem M, Tracey L, Obenauer JC, Panetta JC, Relling MV, Evans WE
In:
Hum Mol Genet (2007) 16(19): 2261-71
Intracellular signaling required for CCL25-stimulated T cell adhesion mediated by the integrin alpha4beta1
Categories:
Transfection
Authors:
Parmo-Cabanas M, Garcia-Bernal D, Garcia-Verdugo R, Kremer L, Marquez G, Teixido J
In:
J Leukoc Biol (2007) 82(2): 380-91
The cytosolic protein talin induces an intermediate affinity integrin alphaLbeta2
Categories:
Transfection
Authors:
Li YF, Tang RH, Puan KJ, Law SK, Tan SM
In:
J Biol Chem (2007) 282(33): 24310-9
DOCK2 is required for chemokine-promoted human T lymphocyte adhesion under shear stress mediated by the integrin alpha4beta1
Categories:
Transfection
Authors:
Garcia-Bernal D, Sotillo-Mallo E, Nombela-Arrieta C, Samaniego R, Fukui Y, Stein JV, Teixido J
In:
J Immunol (2006) 177(8): 5215-25
Human T-Cell Leukemia Virus Type 1 (HTLV-1) and HTLV-2 Use Different Receptor Complexes To Enter T Cells
Categories:
Transfection
Authors:
Jones KS, Fugo K, Petrow-Sadowski C, Huang Y, Bertolette DC, Lisinski I, Cushman SW, Jacobson S, Ruscetti FW
In:
J Virol (2006) 80(17): 8291-302
Vav1 and Rac Control Chemokine-promoted T Lymphocyte Adhesion Mediated by the Integrin 41
Categories:
Transfection
Authors:
Garcia-Bernal D, Wright N, Sotillo-Mallo E, Nombela-Arrieta C, Stein JV, Bustelo XR and Teixido J
In:
Mol Biol Cell (2005) 16(7): 3223-3235
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