NRK

kidney, epithelial

Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
rat
Research Area:
Gastroenterology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T X-001 1e6 43-45% 87-95% Plasmid (general) 5 µg 100 µl I/II/2b
SF DK-101 2e5 30% 66-80% Plasmid (general) 1 µg 20 µl 4D X-Unit
SF DK-101 1e6 41-43% 66-82% Plasmid (general) 5 µg 100 µl 4D X-Unit

Citations

Categories:
Transfection 
Authors:
Guo WH, Wang YL 
In:
Mol Biol Cell (2007) 18(11): 4519-4527 
Categories:
Transfection 
Authors:
Lo KW, Kogoy JM, Pfister KK 
In:
J Biol Chem (2007) 282(15): 11205-12