Astrocyte (NHA), human

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Star-shaped glial cells that provide nutrients, support, and insulation for neurons of the central nervous system.

Cell Type:
Glial Cells
Tissue Origin:
brain
Species:
human
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Research Area:
Cancer Research/Cell Biology
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

Astrocyte Growth Medium

Astrocyte Growth Medium (AGM™) has been developed to support the growth of primary human astrocytes.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 96-DC-104 3e5 85% good Plasmid (general) 400 ng 20 µl 96-well
Neuron, basic T-020 3e6 83% more than 50% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (11)

Palm Fruit Bioactives modulate human astrocyte activity in vitro altering the cytokine secretome reducing levels of TNFa, RANTES and IP-10
Categories:
Primary Cells and Media 
Authors:
Weinberg RP, Koledova VV, Schneider K, Sambandan TG, Grayson A, Zeidman G, Artamonova A, Sambanthamurthi R, Fairus S, Sinskey AJ, Rha C. 
In:
Scientific Reports (2018) 8 (1): 16423 
Astrocyte elevated gene-1 is a novel modulator of HIV-1-associated neuroinflammation via regulation of nuclear factor-?B signaling and excitatory amino acid transporter-2 repression.
Categories:
Transfection 
Authors:
Vartak-Sharma N, Gelman BB, Joshi C, Borgamann K, Ghorpade A 
In:
J Biol Chem (2014) 289(28): 19599-612 
Reactive astrocytes promote the metastatic growth of breast cancer stem-like cells by activating Notch signalling in brain.
Categories:
Primary Cells and Media 
Authors:
Fei Xing1,2, Aya Kobayashi1, Hiroshi Okuda1, Misako Watabe1, Sudha K. Pai1, Puspa R. Pandey1, Shigeru Hirota3, Andrew Wilber1, Yin-Yuan Mo1,2, Brian E. Moore4, Wen Liu1, Koji Fukuda1, Megumi Iiizumi1, Sambad Sharma2, Yin Liu2, Kerui Wu2, Elizabeth Peralta5, Kounosuke Watabe1,2* 
In:
EMBO Mol Med (2013) 5(3): 384-96 
Multicellular self-assembled spheroidal model of the blood brain barrier
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Urich E, Patsch C, Aigner S, Graf M, Iacone R, Freskgård PO. 
In:
Scientific Reports (2013) 3: 1500 
A radial glia gene marker, fatty acid binding protein 7 (FABP7), is involved in proliferation and invasion of glioblastoma cells
Categories:
Primary Cells and Media, Transfection 
Authors:
De Rosa A, Pellegatta S, Rossi M, Tunici P, Magnoni L, Speranza MC, Malusa F, Miragliotta V, Mori E, Finocchiaro G, Bakker A. 
In:
PLoS ONE (2012) 7(12): ePub 
Nonsteroidal anti-inflammatory drug-activated gene (NAG-1/GDF15) expression is increased by the histone deacetylase inhibitor trichostatinA
Categories:
Primary Cells and Media 
Authors:
Yoshioka H1, Kamitani H, Watanabe T, Eling TE 
In:
J Biol Chem (2008) 283(48): 31129-37 
Capsaicin-induced apoptosis of glioma cells is mediated by TRPV1 vanilloid receptor and requires p38 MAPK activation
Categories:
Primary Cells and Media 
Authors:
Amantini C, Mosca M, Nabissi M, Lucciarini R, Caprodossi S, Arcella A, Giangaspero F, Santoni G. 
In:
J Neurochem (2007) 102(3): 977-90 
Human immunodeficiency virus-restricted replication in astrocytes and the ability of gamma interferon to modulate this restriction are regulated by a downstream effector of the Wnt signaling pathwaysignaling pathway
Categories:
Transfection 
Authors:
Carroll-Anzinger D, Kumar A, Adarichev V, Kashanchi F, Al-Harthi L 
In:
J Virol (2007) 81(11): 5864-71 
MEK1/2 inhibitors block basal and TGF-1 stimulated JC virus multiplication
Categories:
Transfection 
Authors:
Ravichandran V, Jensen PN, Major EO 
In:
J Virol (2007) 81(12): 6412-8 
Interactions between c-Jun, Nuclear Factor-1 and JC Virus promoter sequences: Implications for viral tropism
Categories:
Transfection 
Authors:
Ravichandran V, Sabath BF, Jensen PN, Houff SA, Major EO 
In:
J Virol (2006) 80(21): 10506-13 
A novel in vitro system, the integrated discrete multiple organ cell culture (IdMOC) system, for the evaluation of human drug toxicity: comparative cytotoxicity of tamoxifen towards normal human cells from five major organs and MCF-7 adenocarcinoma breast cancer cells.
Categories:
Primary Cells and Media 
Authors:
Li AP1, Bode C, Sakai Y 
In:
Chem Biol (2004) 150(1): 129-36 
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