Hepatocyte, rat

Hepatocyte, Rat, cryopreserved and freshly isolated

Cell Type:
Liver Epith.
Tissue Origin:
liver
Species:
rat
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Suspension

Recommended Media

In 1971, Williams et al. described a procedure for enriching isolated hepatocyte cultures for polygonal epithelial cells and reducing the number of contaminating fibroblasts. This method was a sequential plating technique based on the observation that fibroblast-like cells adhere to a substrate more rapidly than epithelial cells. The isolated epithelial cells resulting from this treatment could then be cultured in a rich medium designated Williams' Medium D. Newborn animals were the source of cells used in these studies, as they were in most studies of liver cell culture. Since newborn liver is not functionally mature, further studies were conducted by Williams and Gunn to explore the possibility of culturing adult liver on a long-term basis. The medium developed during the course of these studies was designated Williams' Medium E. It has been shown to support the growth in long-term culture of adult liver epithelial cells.

Storage = 2ºC to 8ºC

12-761 - without L glutamine
02-019 - without L-glutamine or phenol red

HCM is  a serum-free medium for maintenance of cultures prior to and during experimental procedures.
It is the preferred medium when working with cryopreserved human and animal hepatocytes.

HMM is a serum-free medium that may be used to maintain fresh plated human and animal hepatocytes in culture during testing.

Hepatic media for the plating of fresh and cryopreserved, human and animal, primary hepatocytes. The medium consists of a basal Medium with a supplement.

MP100: Hepatocyte Plating Medium w/Supplement (MP100-1 + MP100-2), 100ml
MP250: : Hepatocyte Plating Medium w/Supplement (MP250-1 + MP250-2), 250 ml

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Hepatocyte, mouse/rat Q-025 4e5 46-58% 68-88% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (13)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Koen YM, Liu K, Shinogle H, Williams TD, Hanzlik RP 
In:
Chemical Research in Toxicology (2016) ePub: ePub 
Categories:
 
Authors:
Ding C1, Li Y2, Guo F3, Jiang Y3, Ying W3, Li D3, Yang D3, Xia X3, Liu W3, Zhao Y3, He Y4, Li X3, Sun W3, Liu Q3, Song L3, Zhen B3, Zhang P3, Qian X5, Qin J6, He F7. 
In:
Mol Cell Proteomics (2016) 15(10): 3190-3202 
Categories:
 
Authors:
Chan HF1,2, Zhang Y1,2, Leong KW1,2 
In:
Other (2016) 12(20): 2720-2730 
Categories:
Primary Cells and Media 
Authors:
Khetani SR1, Berger DR2, Ballinger KR3, Davidson MD2, Lin C2, Ware BR2 
In:
J Lab Automation (2015) 20(3): 216-250 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhang LW, Koci J, Jeffery B2, Riviere JE, Monteiro-Riviere NA 
In:
Food Chem Toxicol (2015) 78: 105-15 
Categories:
 
Authors:
Bachmann A1, Moll M2, Gottwald E3, Nies C4, Zantl R5, Wagner H6, Burkhardt B7, Sánchez JJ8, Ladurner R9, Thasler W10, Damm G11, Nussler AK12. 
In:
Other (2015) 4(1): 64-83 
Categories:
 
Authors:
Einolf HJ1, Chen L2, Fahmi OA3, Gibson CR4, Obach RS3, Shebley M5, Silva J6, Sinz MW7, Unadkat JD8, Zhang L9, Zhao P9 
In:
Other (2014) 95(2): 179-188 
Categories:
 
Authors:
Prueksaritanont T1, Tang C. 
In:
Other (2012) 14(3): 410-419 
Categories:
 
Authors:
Suzuki H1, Inoue T, Matsushita T, Kobayashi K, Horii I, Hirabayashi Y, Inoue T. 
In:
Other (2008) 28(2): 227-236 
Categories:
Transfection 
Authors:
Li X, Pandak WM, Erickson SK, Ma Y, Yin L, Hylemon P, Ren S 
In:
J Lipid Res (2007) 48(12): 2587-96 
Categories:
 
Authors:
Spotorno VG1, Hidalgo A, Barbich M, Lorenti A, Zabal O. 
In:
Cytotechnology (2006) 51(2): 51-56 
Categories:
 
Authors:
Crespi CL1, Chang TK, Waxman DJ. 
In:
Methods Mol Biol (2006) 320: 115-119 
Categories:
 
Authors:
Tang C1, Lin JH, Lu AY 
In:
Drug Metabolism and Disposition (2005) 33(5): 603-613 
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